Construction and in vitro cytotoxicity of composite scaffolds by polyethylene glycol modification of acellular porcine aortic valves

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ZHOU Jian-liang(Dopartment of Cardiothoracic Surgery, The Second Affiliated Hospital, Nanchang University, Nanchang 330006, China)
ZOU Ming-hui()
ZHOU Cheng()
LU Cui-fen()
SHI Jia-wei()
DONG Nian-guo()
Journal Title:
Chinese Journal of Biomedical Engineering
Volume 18, Issue 01, 2012
Key Word:
Acellular aortic valves;Polyethylene glycol;Crosslink;Cytotoxicity

Abstract: Objective To modify acellular porcine aortic valve scaffolds using polyethylene glycol (PEG) and to assess their cytotoxicity in vitro. Methods Dendritic PEG derivatives with acryloyl functional group were synthesized followed by introduction of thiol groups into acellular porcine aortic valves and cross- linking with PEG via Michael addition reaction.The biomechanical properties prior to and after PEG modification were compared.The extracts were divided into three groups:decellularized valve group,PEG - modified decellularized valve group, and control group (RPMI 1640 medium ).The impact of composite scaffolds extractant on human umbilical vein endothelial cells proliferation and in vitro cytotoxicity were assessed based on CCK - 8 assay.Results Modification of acellular porcine aortic valves using PEG occurred under mild reaction conditions and showed well-defined outcomes.Composite valve scaffolds had a markedly higher stretch- proof capacity than acellular porcine aortic valves [ (7.53±0.29) MPa vs (5.65±0.24) MPa,P<0.05] but did not differ statistically from natural valves [ (7.53 ±0.29)MPa,P>0.05].Cytotoxicity of composite scaffolds and acellular porcine valves were both graded as zero.Human umbilical vein endothelial cells cultured with medium containing composite valve scaffold extractants appeared in normal morphology and active proliferation.Conclusion PEG modification significantly improves the mechanical properties of acellular porcine aortic valves and reduces the cytotoxicity of composite scaffolds,thereby providing prospects for further researches.

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