Effects of bone marrow mesenchymal stem cells transfected with lentivirus in spinal fusion
- Author:
- No author available
- Journal Title:
- Chinese Journal of Biomedical Engineering
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1674-1927.2012.01.004
- Key Word:
- 慢病毒;骨髓间充质干细胞;脊柱融合;骨形态发生蛋白;Lentivirus;Bone marrow mesenchymal stem cell;Spine fusion;Bone morphogenetic protein
Abstract： Objective To assess the spinal fusion in rats induced by bone marrow mesenchymal stem cells (BMSC) transfected with lentivirus.Methods Alkaline phosphatase (ALP) staining and ALP activity were determined in BMSC transfected with lentivirus encoding BMP-2 that were implanted to rat model of spinal fusion.At week 8,all rats were sacrificed.Thereafter,measurements of manual mechanics,Micro-CT scanning and histological analysis were performed.Results ALP staining appeared more satisfactory in BMSC transfected with lentivirus as compared with untransfected cells (3.2±0.4 vs 1.1 ± 0.2,P＜0.05).At week 8,spine bony fusion was achieved in all the transfected rats,as indicated by radiographic evaluation,Micro-CT and manual mechanics.In contrast,spinal fusion was absent in untransfected rats.Conclusion Lentivirus-transfected BMSC may achieve well-induced spinal fusion,and lentivirus vectors may offer the application of spinal fusion.
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