Effects of nicotine on intracellular Ca2+ concentration and transient receptor potential channels mRNA expression in rat airway smooth muscle cells

( views:231, downloads:0 )
Author:
TIAN Yan(Guangzhou Institute of Respiratory Diseases,The First Affiliated Hospital,Guangzhou Medical College,Guangzhou 510182,China)
WANG Jian(Guangzhou Institute of Respiratory Diseases,The First Affiliated Hospital,Guangzhou Medical College,Guangzhou 510182,China)
RAN Pi-xin(Guangzhou Institute of Respiratory Diseases,The First Affiliated Hospital,Guangzhou Medical College,Guangzhou 510182,China)
Journal Title:
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
Issue:
Volume 17, Issue 02, 2011
DOI:
10.3760/cma.j.issn.1674-1927.2011.02.003
Key Word:
Nicotine;Transient receptor potential channel;Myocytes,smooth muscle;Intracellular Calcium-sensing proteins;Muridae

Abstract: Objective To study the changes of intracellular Ca2+ concentration and transient receptor potential channels 1 and 6(TRPC1,TPRC6)mRNA expression in rat airway smooth muscle cells (ASMC) after nicotine intervention.Methods Various concentrations of nicotine(10 μg/L,20 μg,L,50μg/L,100μg/L)were used to stimulate rat airway smooth muscle cells.The intracellular Ca2+ concentrations after nicotine intervention for 24 and 48 hours were measured by Incyte system.The total RNA was extracted from rat ASMC.and reversely transcribed into cDNA.The real-time PCR method was used to analyze the changes in TRPC1 and TRPC6 mRNA expression.Results The groups treated with different concentrations of nicotine(20 μg/L,50μg/L,100 μg/L)for 24 or 48 hours showed increases in intracellular Ca2+concentration when compared with control[(117.99±19.39),(122.89±17.91),(124.70±17.93)nmol/L vs (85.85±12.60)nmol/L;(142.07±18.99),(162.27±19.91),(207.30±26.56)nmol/L vs(98.04±2.39)nmol/L;P<0.05].In addition,relative expressions of TRPC1 and TPRC6 mRNA in rat ASMC also increased significantly compared with controls(P<0.05).The levels of intracellular Ca2+ concentration and TRPC6mRNA were significantly higher with 48 h nicotine(100 μg/L,L)intervention than as found with 24 h nicotineintervention.Conclusion Nicotine may increase the intracellular Ca2+ concentration by up-regulating the expression of TRPC1 and TPRC6 in rat ASMC.

  • [1]Macnee W.Pathogenesis of chronic obstructive pulmonary disease.Clin Chest Med,2007,28:479-513.
  • [2]Jindal SK,Aggarwal AN,Chaudhry K,et al.A multicentric study on epidemiology of chronic obstructive pulmonary disease and its relationship with tobacco smoking and environmental tobacco smoke exposure.Indian J Chest Dis Allied Sci,2006,48:23-29.
  • [3]Hakonarson H,Grunstein MM.Regulation of second messengers associated with airway smooth muscle contraction and relaxation.Am J Respir Crit Care Med,1998,158(5 Pt 3):115-122.
  • [4]Hogg JC.Pathology of asthma.JAllergy Clin Immunol,1993,92:1-5.
  • [5]Sweeney M,McDaniel T,Platoshyn O,et al.Role of capacitative Ca2 + entry in bronchial contraction and remodeling.J Appl Physiol,2002,92:1594-1602.
  • [6]Huang GN,Zeng WZ,Kim JY,et al.STIM1 carboxyl-terminus activates native SOC,I (crac) and TRPC1 channels.Nat Cell Biol,2006,8:1003-1010.
  • [7]Golovina VA,Platoshyn 0,Bailey CL,et al.Upregulated TRP and enhanced capacitative Ca2+ entry in human pulmonary artery myocytes during proliferation.Am J Physiol Heart Ciro Physiol,2001,280:746-755.
  • [8]Sweeney M,Yu Y,Platoshyn O,et al.Inhibiton of endogenous TRPC1 decreases capacitative Ca2+ entry and attenuates pulmonary artery smooth muscle cell proliferation.Am J Physiol Lung Cell Mol Physiol,2002,283:144-155.
  • [9]Yu Y,Sweeney M,Zhang S,et al.PDGF stimulates pulmonary vascular smooth muscle cell proliferation by regulating TRPC6 expression.Am J Physiol Cell Physiol,2003,284:316-330.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn