Abstract： Objective To investigate the toxicity effects of induced breeding with various ion concentrations of cadmium on the lysosomal membrane of coelomocytes in Pheretima aspergillum. Methods Seventy healthy Pheretima aspergilla were selected and randomized into 7 groups (n=10 each) according to different levels of cadmium ion in the soils [0.28 mg/kg (control group) , 3.28 mg/kg, 6.28 mg/kg, 12.28 mg/kg, 18.28 mg/kg, 24.28 mg/kg and 30.28 mg/kg, respectively] and were bred with the contaminating cadmium ions for 14 days. On day 7 and day 14, the morphological changes in coelomocytes were studied and neutral red retention time (NRRT) of coelomocyte lysosome was determined in Pheretima aspergilla.Results High levels of cadmium ion were associated with apparent damages to the coelomocyte lysosome in Pheretima asperillum. NRRT decreased along with increasing levels of cadmium ion by (71.3±2.4) min ,(60.5±1.6) min, (55.1±2.9) min, (51.9±3.6) min, (46.0±2.5) min, (38.8±1.8) min and (34.2±2.0) min in the groups containing 0.28 mg/kg (control group), 3.28 mg/kg, 6.28 mg/kg, 12.28 mg/kg, 18.28 mg/kg,24.28 mg/kg and 30.28 mg/kg cadmium on day 7. Compared with the control group, NRRT of other groups was significantly shorter on day 7 and day 14 (all P＜0.05). Conclusion NRRT can be used to evaluate the toxicity effects in Pheretima asperillum exposed to soils contaminated by metal cadmium.