Expressions of CD95 and Bcl-2 in bone marrow haemopoietic cells from mice with aplastic anemia

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Author:
HONG Jie-min(Central Laboratory, Guangzhou Red Cross Hospital, The Fourth Affiliated Hospital, Jinan University Medical College, Guangzhou 510220, China)
LI Qing-mei(Central Laboratory, Guangzhou Red Cross Hospital, The Fourth Affiliated Hospital, Jinan University Medical College, Guangzhou 510220, China)
ZHOU Yong-xia(Central Laboratory, Guangzhou Red Cross Hospital, The Fourth Affiliated Hospital, Jinan University Medical College, Guangzhou 510220, China)
Journal Title:
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
Issue:
Volume 15, Issue 04, 2009
DOI:
10.3760/cma.j.issn.1674-1927.2009.04.012
Key Word:
Anemia;aplastie;Apoptosis;Antigens;CD34;Antigens;CD95;Bcl-2 proto-oncogene proteins

Abstract: Objective To investigate the expressions of CD95 and Bcl- 2 in bone marrow haemopoietic cells from mice with aplastic anemia and their relationship. Methods The mouse model of aplastic anemia was established by irradiation and immuno-mediation. Flow cytometry was employed to analyze the CD34+ cells, CD45+ cells, as well as expressions of surface CD95 and intracellular Bcl-2. Results The ratio of CD34+ cells with low expresson of CD45 (CD34+ CD45low) cells in the bone marrow was significantly lower in model group than that in control group [(0.85 ± 0.19)% vs (1.21±0.28)%, P< 0.05]. The ratio of CD95 expression in model group was increased significantly on nucleated cells compared to control group [(66.30±7.68)% vs (42.78±15.12)%], on CD34+CD45+ cells [(69.21±9.36)% vs (22.31± 3.33)%], and on CD34+CD45low cells [(53.70±16.46)% vs (7.68±4.42)%], respectively, all P<0.01. The ratio of Bcl-2 expression appeared comparable on different cells except on CD34+CD45low cells, which was (7.60±2.40)% in the model group and (0.70±0.64)% in control group with significant difference (P<0.05). The expressions of CD95 was not correlated with Bcl-2 in control group, but CD95 was negatively correlated with Bcl-2 on CD34+CD45+ celIs (r=-0.93, P<0.05)and CD34+CD45low cells (r=-0.81, P<0.05) of model group. Conclusions Mice with aplastic anemia showed lower ratio of haemopoietic cells, higher expression ratio of CD95 on nucleated and CD34+ cells in bone marrow, and obvious apoptosis. Regulation targeting at different markers of apoptosis may be helpful for effective treatment of aplastic anemia.

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