Protective effects of adenosine preconditioning against myocardial ischemia reperfusion injury in rats with different route of administration

( views:, downloads: )
Author:
CHEN Guang-xian(Department of Cardiac Surgery,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China)
WU Zhong-kai(Department of Cardiac Surgery,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China)
TANG Bai-yun(Department of Cardiac Surgery,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China)
LIU Hai(Department of Cardiac Surgery,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China)
Journal Title:
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
Issue:
Volume 14, Issue 06, 2008
DOI:
Key Word:
Adenosine;Ischemic preconditoning;Reperfusion injury;Myocardialischemia;Nuclear factor kappa B;Cardiac troponin T;Rats

Abstract: Objective To evaluate the effect on myocardial protection of adenosine preconditioning in different route of administration through right jugular vein and left ventricle.Methods 48 SD rats were randomly divided into ischemia reperfusion group(blank control),ischemic preconditioning group(IP,positive control),adenosine venous infusion group,adenosine in ventricular group,normal saline(NS)venous infusion group(negative control I)and NS in ventricular group(negative control Ⅱ).The ischemia reperfusion rats model were established in vivo,and then changes of heart function,serum cardiac troponin T (cTnT),superoxide dismutase(SOD),malondialdehyde(MDA)and expression of nuclear factor KB(NF-kB) were observed.Results SOD in IP[(208.63±23.88)U/ml],adenosine venous infusion group [(178.27±11.56) U/ml]and adenosine in ventricular group[(191.31±28.14)U/ml]were significantly higher than that in the ischemia reperfusion group[(145.05±23.18)U/ml](P<0.05),cTnT,MDA and expression of NF-kB were lower than that in the ischemia reperfusion group (P<0.05).Heart function was significantly better than that in the ischemia reperfusion group(P<0.05);SOD in adenosine in ventricular group was significantly higher than that in adenosine venous infusion group(P<0.05).cTnT,MDA and expression of NF-kB were lower than that in adenosine venous infusion group (P<0.05).Conclusion Adenosine preconditioning may mimic protective effect of ischemic preconditioning. The effect on myocardial protection of adenosine in ventricular group was better than that of adenosine venous infusion group.

  • [1]Takahama H,Minamino T,Asanuma H,et al.Prolonged targeting of ischemic/repeffused myocardium by liposomal adenosine augments eardioprotection in rats.J Am Coil Cardiol,2009,53:709-717.
  • [2]Wu ZK,Laurikka J,Saraste A,et al.Cardiomyoeyte apoptosis and ischemic preconditioning in open heart operations.Ann Thorac Surg,2003,76:528-534.
  • [3]Henning S,Evangelos G,Simon F,et al.Cardiac Troponin T at 96 hours after acute myocardial infarction correlates with infarct size and cardiac function.J Am Coll Cardio,2006,8:2192-2194.
  • [4]Moss NC,Tang RH,Willis M,et al.Inhibitory kappa B kinasebeta is a target for specific nuclear factor kappa B-mediated delayed eardioprotection.J Thorac Cardiovasc Surg,2008,136:1274-1279.
  • [5]D'Souza KM,Petrashevskaya NN,Merrill WH,et al.Inhibition of protein kinase C alpha improves myocardial beta-adrenergic receptor signaling and ventricular function in a model of myocardial preservation.J Thorac Cardiovasc Surg,2008,135:172-179.
  • [6]Xi L,Das A,Zhao ZQ,et al.Loss of myocardial ischemic postconditioning in adenosine A1 and bradykinin B2 receptors gene knockout mice.Circulation,2008,118:32-37.
  • [7]Lu K,Otani H,Yamamura T,et al.Protein kinase C isoformdependent myocardial protection by ischemic preconditioning and potassium cardioplegia.J Thorac Cardiovasc Surg,2001,121:137-148.
  • [8]Bright R,Mochly-Rosen D.The role ofprotein kinase C in cerebral ischemic and reperfusion injury.Stroke,2005,36:2781-2790.
  • [9]Zhao JL,Yang YJ,Pei WD,et al.Intravenous adenosine reduces myocardial no-reflow by decreasing endothelin-1 via activation of the ATP-sensitive K+ channel.Acta Cardiol,2008,63:355-359.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn