Experimental study on repairing pelvic autonomic nerve injury by bone marrow mesenchymal stem cells and autologous peritoneum

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Author:
SONG Xin-ming(Department of Gastrointestinal and Pancreatic Surgery,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China.)
ZHANG Li(Department of Gastrointestinal and Pancreatic Surgery,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China.)
LI Ming-zhe(Department of Gastrointestinal and Pancreatic Surgery,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China.)
CHEN Zhi-hui(Department of Gastrointestinal and Pancreatic Surgery,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China.)
HE Yu-long(Department of Gastrointestinal and Pancreatic Surgery,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China.)
CAI Shi-rong(Department of Gastrointestinal and Pancreatic Surgery,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China.)
HUANG Yi-hua(Department of Gastrointestinal and Pancreatic Surgery,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China.)
()
Journal Title:
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
Issue:
Volume 14, Issue 03, 2008
DOI:
Key Word:
Bone marrow;Mesenchymal stem cells;Pelvic autonomic nerve;Injury;Transplantation

Abstract: Objective To evaluate the effect of using the bridge consisting of bone marrow mesenchymal stem cells(BMSCs) and autologous peritoneum to repair the injured pelvic autonomic nerve. Methods Injury models were made on beagle dogs and divided into three groups: collagen protein sponge and BMSCs were inseted in peritoneal canal to bridge the gap in experimental group, BMSCs was replaced by saline in control group, and autologous nerve was cutted previously and inversed 180° to bridge the gap in normal control group. Specimens were obtained after 12 weeks, and observed by HE and NF immunohistochemical staining. The chosen features was measured using image analytic system. Uhramierostructure in experimental group was observed by transmission electron microscope. Results The sum of regenerative nerve fiber (1742±185 vs 1131±262, P<0.01 ), density [ (168±14) (104μm2)-1 vs (124±17) (104μm2)-1, P<0.01 ], diameter [ (3.83±0.22) μm vs (3.28±0.41) μm, P<0.05] and area percent (0.32±0.07 vs 0.21±0.08, P<0.05 ) were significantly different between experimental group and control group respectively, and there were no significant differences between experimental group and normal control group ( P>0.05 ). The morphous of regenerative nerve fiber was near normal and all constitution was clear in experimental group. Conclusion The regenerative nerve is grown well. This bridge consisting of BMSCs and autologous peritoneum to repair the injured pelvic autonomic nerve. This method is feasible,and the result is the same as using autologous nerve to transplant.

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