Dynamic changes of microscopical structure and NOS expression in rat lung tissues under simulated weightlessness

( views:276, downloads:1 )
Author:
WANG De-sheng(Department of Pathophysiology,College of Veterinary Medicine,China Agricultural University,Beijing 100094,China)
YUAN Ming()
LI Zhi-li()
WANG Hui-juan()
LI Tian-zhi()
LIU Chang-ting()
QIAO Jian(Department of Pathophysiology,College of Veterinary Medicine,China Agricultural University,Beijing 100094,China)
Journal Title:
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
Issue:
Volume 14, Issue 01, 2008
DOI:
Key Word:
Weightlessness simulation;Lung;Pathology;Nitricoxide synthase

Abstract: Objective To observe dynamic changes of microscopical structure and nitric oxide synthase(NOS) expression in rat lung tissues under simulated weightlessness,and to collect the data for studies of the adaptive mechanism of local regulation in lung tissues. Methods Wistar male rats were -30° tail suspended to simulate the physiological effects of weightlessness. The microscopical structure,constitutive NOS(cNOS)and inducible NOS(iNOS)expression of rat lung tissues in control group(Con),7-day tail suspension group(TS7) and 14-day tail suspension group(TS14) were respectively observed with routine microscope and immunohistochemistry. Results The solid edema of lungs appeared in TS7 rats,accompanied with lymphocyte soakage in bronchial mucous membranes,red blood cells in alveolus and alveolar amalgamation. The pathological change was more obvious in the TS14 group than that in TS7 group,accompanied with more alveolar amalgamation,more red blood cells in alveolus and thicker alveolar walls. The cNOS expression regions of lung tissues in three groups were mostly the bronchial epithelium,vascular endothelial cells and vascular smooth muscle cells,but there were no significant differences among all groups. The iNOS expression of the TS7 and TS14 rat lung tissues increased significantly as compared with Con rats,which was more obvious in vascular endothelial cells of TS14 than TS7.Conclusion The morphological changes in lung tissues induced by simulated weightlessness are mainly due to the up-regulation of iNOS expression in pulmonary circulation.

  • [1]沈羡云.航天中的心血管问题.航天医学与医学工程,1992,5:295-300.
  • [2]West JB.Microgravity and the lung.Physiologist,1991,34:s8-s10.
  • [3]汪德生,孙磊,梁文彬,等.模拟失重对肺动脉和胸主动脉的影响.中国应用生理学杂志,2003,19:269-273.
  • [4]陈杰,马进,丁兆平,等.一种模拟失重影响的大鼠尾部悬吊模型.空间科学学报,1993,13:159-162.
  • [5]Engel LA.Effects of microgravity on the respiratory system.J Appl Physiol,1991,70:1907-1911.
  • [6]Verbanck S,Larsson H,Linnarsson D,et al.Pulmonary tissue volume,cardiac output,and diffusing capacity in sustained microgravity.J Appl Physiol,1997,83:810-816.
  • [7]汪德生,向求鲁,沈羡云,等.7天头低位卧床对心肺循环功能的影响.航天医学与医学工程,1998,11:177-180.
  • [8]汪德生,袁敏,李志利,等.尾部悬吊大鼠胸主动脉、肺动脉超微结构变化的动态观察.现代临床医学生物工程学杂志,2006,12:416-419.
  • [9]汪德生,孙磊,任维,等.尾部悬吊大鼠胸主动脉、肺动脉内皮细胞一氧化氮合酶mRNA表达的动态变化.航天医学与医学工程,2001,14:318-322.
  • [10]Atkov OY,Bednenko VS.Hypokinesia and weightlessness:clinical and physiologic aspects.USA Connecticut Madison:International Universities Press,1992:24-295.
  • [11]Shykoff BE,Farhi LE,Olszomka AJ,et al.Cardiovascular response to submaximal exercise in sustained microgravity.J Appl Physiol,1996,81:26-32.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn