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Correlation of cytokine expression in bone marrow-derived mesenchymal stem cells with activity of systemic lupus erythematosus
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1008-634X.2006.06.003
- Key Word:
- 红斑狼疮,系统性;间充质干细胞;细胞因子
Abstract： 目的 探讨系统性红斑狼疮(SLE)患者骨髓间充质干细胞(MSCs)细胞因子分泌及其与疾病活动的相关性.方法 采用密度梯度离心和贴壁分离法对11例SLE患者和6例正常人骨髓MSCs进行分离培养,流式细胞术鉴定MSCs.取P2代细胞,半定量RT-PCR检测SLE患者骨髓MSCs白细胞介素-6(IL-6),IL-7,IL-11,巨噬细胞集落刺激因子(M-CSF)和干细胞因子(SCF)的表达.结果 SLE患者MSCs均表达CD29、CD44和CD105,不表达CD14、CD34、CD45和HLA-DR.两组P2代MSCs均表达IL-6、IL-7、IL-11、M-CSF和SCF.SLE患者组MSCs IL-6、IL-7表达降低(P＜0.01),IL-7的表达和SLE的活动评分(SLEDAI)呈负相关(r=-0.891,P＜0.05).结论 SLE患者MSCs细胞因子分泌异常,可能与SLE血液系统损害及病情活动相关.
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