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Detection of Mutation Frequencies in the CYP3A4 Exon 9 Gene with PCR-RFLP in Health Individuals
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1008-634X.2005.05.005
- Key Word:
- 聚合酶链反应;多态性;限制性片段长度;CYP3A4
Abstract： 目的建立一种简便、准确、实用的人CYP3A4第9外显子基因突变频率的检测方法,并了解汉族人CYP3A4第9外显子的分布特点.方法应用聚合酶链反应(PCR)特异性扩增人CYP3A4第9外显子基因序列,扩增产物用限制性内切酶HinfⅠ酶切,琼脂糖凝胶电泳后,观察酶切位点的限制性片段长度多态性(RFLP)图谱.结果运用PCR-RFLP法检测了92名汉族人CYP3A4第9外显子基因点突变,其中野生型纯合子频率为85.9%,杂合子频率为14.1%,突变型纯合子频率为0.突变等位基因频率为0.0706.结论该方法简便、快速、准确,适合于一般实验室检测及大规模的人群调查,汉族人CYP3A4第9外显子也存在相同的突变位点.
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