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Calcium Signal in Cardiomyocytes Hypertrophy Induced by Neuropeptide Y in Rats
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1008-634X.2005.03.006
- Key Word:
- 神经肽;Y;心肌细胞肥大;钙信号
Abstract： 目的探讨钙依赖的信号转导途径在神经肽Y诱导心肌细胞肥大中的作用.方法用NPY刺激Wistar乳鼠心肌细胞,并用Ca2+/CaM依赖的钙调神经磷酸酶(CaN)的特异性抑制剂环胞素A(CsA)加以干预.应用3H-Leu掺入量法测定心肌细胞蛋白质合成速率, 用Fluo3-AM荧光标记细胞内游离钙离子, 观察不同作用时限内心肌细胞胞浆及核内钙离子浓度变化.结果①心肌细胞3H-Leu掺入量测定:与对照组相比,NPY10nM组3H-Leu掺入量有所增高,但与对照组比无显著差异,而NPY100nM 组心肌细胞3H-Leu掺入量较对照组明显增高(p<0.05).CsA组和对照组相比无显著差异.②加入100nmol/L NPY即刻至10min内,心肌细胞胞浆及核内钙含量均无显著差异.经100nmol/L NPY刺激24h后,心肌细胞胞浆及核内钙含量明显高于对照组(p<0.001,p<0.001).结论 NPY刺激心肌细胞蛋白质合成增加,提示NPY可诱导心肌细胞肥大;CaN抑制剂CsA可阻断NPY上述效应,说明Ca2+/CaM依赖的CaN信号途径在其中起重要作用.较长时间的NPY刺激可导致心肌细胞胞浆及核内钙Ca2+浓度增加,这可能是活化CaN信号途径的始动环节.
- [1]陈敏生,刘建康,黄少华.神经肽Y对培养的血管平滑肌细胞增殖的影响[J].1999,(3).
- [2]曾春雨,刘光耀,王旭开.培哚普利对老年人心脏保护性作用与神经肽Y的关系[J].1998,(2).
- [3]Schluter KD,Goldberg Y,Piper HM,etc.Intracellular signaling leads to the hypertrophic effect of neuropeptide Y.[J].1998,275(5 Pt.1).
- [4]Bell D,Balasubramaniam A,McDermott BJ,etc.Induction of hypertrophic responsiveness of cardiomyocytes to neuropeptide Y in response to pressure overload.[J].2002,303(2).
- [5]B C, Millar,K D, Schlüter,X J, Zhou,etc.Neuropeptide Y stimulates hypertrophy of adult ventricular cardiomyocytes.[J].1994,266(5 Pt 1).
- [6]Nagai R,Zou Y,Hayashi D,etc.Ca2+/calmodulin-dependent kinase II and calcineurin play critical roles in endothelin-1-induced cardiomyocyte hypertrophy.[J].2000,275(20).
- [7]J D, Molkentin,J R, Lu,C L, Antos,etc.A calcineurin-dependent transcriptional pathway for cardiac hypertrophy.[J].1998,93(2).