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4-Aminopyridine-Induced Increases in Intracellular Free Ca2+ in Cultured Mouse Cortical Neurons
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1008-634X.2005.02.006
- Key Word:
- 4-氨基吡啶;细胞内游离钙离子浓度;谷氨酸;共聚焦技术;皮层神经细胞
Abstract： 目的研究4-氨基吡啶(4-AP)对体外培养的皮层神经元细胞内游离钙浓度([Ca2+]i)的影响,了解4-AP的药理学作用机制.方法荧光探针Fluo-3-AM标记体外培养的皮层神经细胞内游离钙后,用共聚焦显微镜观察记录4-AP及L型谷氨酸对小鼠原代培养的皮层神经元[Ca2+]i的影响.结果 4-AP与谷氨酸均能提高[Ca2+]i,两者峰值与持续时间存在差异,共同作用于细胞时的上升曲线与单用谷氨酸时相仿.结论 4-AP的药理作用机制可能与提高神经细胞[Ca2+]i有关,其中机制与兴奋性氨基酸的[Ca2+]i升高作用可能不同.
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