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The Expression of Neuropilin-1 Gene in Bone Marrow Stromal Cells from Patients with CML and Normal Individuals
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1008-634X.2004.01.005
- Key Word:
- neuropilin-1 基因;慢性粒细胞性白血病;RT-PCR
Abstract： 目的了解Neruophlin-1基因在慢性粒细胞白血病(CML)骨髓基质细胞中的表达情况.方法收集14例CML和20例正常对照骨髓标本,分离单个核细胞.进行体外长期培养,收集贴壁细胞(骨髓基质细胞).利用RT-PCR方法分析两组骨髓基质细胞中的cDNA,了解Neuropilin-1 基因表达情况.结果建立了CML和正常人骨髓基质细胞培养方法,CML骨髓基质细胞中的Neuropilin-1基因表达率(50%)明显低于正常对照(85%)(p<0.05).结论参与调控骨髓基质细胞的Neuropilin-1基因可表达于大部分正常人和部分CML患者骨髓基质细胞中,Neuropilin-1基因在部分CML骨髓基质细胞中的表达缺失可能与其调节造血功能异常有关.
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