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The Expression of Transcription Factor GATA-1 and GATA-2 Genes in Bone Marrow Stromal Cells from Patients with Aplastic Anemia or Normal Controls
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1674-1927.2003.05.002
- Key Word:
- 转录因子;GATA基因;骨髓基质细胞;再生障碍性贫血;RT-PCR-ELISA
Abstract： 目的探讨转录因子GATA-1和GATA-2基因在再障和正常骨髓基质细胞中表达的情况.方法体外扩增培养骨髓基质细胞,运用RT-PCR-ELISA方法检测GATA-1和GATA-2基因的表达情况,并对其相对表达水平进行半定量比较.结果 GATA-1和GATA-2基因在正常和再障的骨髓基质细胞中均有一定的表达.再障的骨髓基质细胞中GATA-1的表达水平较正常低;而GATA-2的表达水平与正常相比无显著性差异.正常和再障的骨髓基质细胞中均以GATA-2的表达占优势.结论转录因子GATA-1和GATA-2基因可在正常和再障骨髓基质细胞中表达,并可能影响骨髓微环境的造血调控作用,对再障等血液疾病的发生发展起一定的作用.
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