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The Experiment Study of Double Nuclides Labeling Cartilage Link Protein
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1674-1927.2003.03.005
- Key Word:
- 125I 99Tcm-CLP 125I-CLP-99Tcm 制备
Abstract： 目的探讨双核素标记软骨链蛋白(CLP)的制备方法.方法采用99TcmO4-、Na125I放射源和CH-T、Iodogen 、SnC12和2-ME四种方法制备99Tcm-CLP、125I-CLP 和125I-CLP-99Tcm放射性药物.结果四种方法获得的125I、99Tcm-CLP、125I-CLP-99Tcm标记率均可达到90%以上,体外结合活性KD分别为8.14、9.01、1.12 、1.07(×108mol/L);125I-CLP-99Tcm为106～107 mol/L;将125I-CLP 和99Tcm-CLP配体混合获得的125I-CLP-99Tcm KD为5.63×108mol/L.结论用Iodogen和SnCl2方法制备125I-CLP 和99Tcm-CLP探针,体外按1∶1混合获得的125I-CLP-99Tcm双标记物可有效减轻氧化剂的损伤作用.
- [1]张汝森,刘长征.99mTc直接法标记抗体的3个常用还原剂的评价[J].2000,(5).
- [2]强永刚,廖永华.软骨链蛋白的分离提取及小鼠体内的示踪动力学[J].2001,(5).
- [3]Aktolun, C.,Ussov, W. Y.,Arka, A.,etc.Technetium-99m and indium-111 double labelling of granulocytes for kinetic and clinical studies[J].1995,22(4).
- [4]T, Shibutani,K, Imai,A, Kanazawa,etc.Use of hyaluronic acid binding protein for detection of hyaluronan in ligature-induced periodontitis tissue.[J].1998,33(5).
- [5]B A, Rhodes,P O, Zamora,K D, Newell,etc.Technetium-99m labeling of murine monoclonal antibody fragments.[J].1986,27(5).