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Construction of an Eukaryotic Expression Vector Inserted HBsAg Gene
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1674-1927.2003.01.004
- Key Word:
- 肝炎病毒;乙型;S基因;构建
Abstract： 目的构建HBsAg真核表达质粒.方法用PCR的方法从质粒pEcob6中扩增出HBsAg基因,并定向克隆到真核表达质粒pcDNA3.1(+),构建成重组质粒pcDNA3.1-S;然后用限制性内切酶消化和DNA序列测定鉴定.结果经酶切和DNA序列测定鉴定,证实重组质粒构建正确.结论真核表达质粒pcDNA3.1-S构建成功.
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