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Effect of Wild Type p53 Gene on Growth Inhibition in Lung Cancer Cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1674-1927.2002.04.008
- Key Word:
- 肺癌;p53
Abstract： 目的探讨外源性野生型p53对肺癌细胞生长的抑制作用. 方法通过向肺癌细胞中分别转染携带有荧光蛋白基因的野生型P53基因和突变型P53基因,而后用荧光显微镜观察荧光蛋白表达;中性红和MTT染料摄入法测定细胞生长活性. 结果 p53蛋白与绿色荧光蛋白之融合蛋白表达定位在胞浆和胞膜;经中性红和MTT两种方法测定,外源性野生型p53对肺癌细胞生长具有抑制作用;OD值分别为 0.71 ±0.081(OD540)和0.65±0.059(OD490),均低于对照组(p<0.05). 结论 p53外显子5～8可在肺癌细胞中获得表达并对细胞生长活性有抑制作用.
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