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Expression Diversity and Influence of Heat-shock Transcription Factor 1 in Breast Cancer Cell Line MCF-7 before and after Hyperthermia or Chemotherapy
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1674-1927.2002.04.006
- Key Word:
- 热休克因子;热休克蛋白;多药耐药;热/化疗;乳腺癌
Abstract： 目的探讨乳腺癌细胞株MCF-7经热处理及应用阿霉素前后热休克因子(HSF1)的表达差异及其对热休克蛋白(HSP70)以及P-糖蛋白表达的影响. 方法分别给予乳腺癌细胞株MCF-7非致死温度的热冲击43℃1小时、45℃15分钟以及阿霉素处理,继续培养4小时后收获细胞,进行免疫细胞化学染色,观察热/化疗前后热休克因子、热休克蛋白以及P-糖蛋白的表达情况. 结果乳腺癌细胞株MCF-7经上述处理后三组细胞的HSF1、HSP70较处理前蛋白表达有明显增高,43℃组、化疗组P-糖蛋白表达较处理前有明显增高,但45℃组P-糖蛋白的表达在热疗前后无明显变化. 结论经热疗或化疗后的乳腺癌细胞株MCF-7中热休克因子表达增高,并能促进热休克蛋白70及P-糖蛋白的表达;热疗可导致化疗耐药的产生.
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