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Experimental Study on Effection of Cerebral Cell's Expression of Bcl-2 Gene by Polygonum Multiflo rum Thunb
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1674-1927.2002.02.002
- Key Word:
- 何首乌;脑细胞;Bcl-2基因
Abstract： 目的在基因水平探求何首乌抗衰老的机制. 方法①Wistar大鼠40只,分成何首乌药物组(W1组)20只和对照组(W2组)20只,取大鼠的脑组织切片进行免疫组化检测,比较两组Bcl-2基因表达;②取长爪沙鼠50只随机分5组,单纯何首乌灌药组(M1),单纯手术组(M2),建立急性脑缺血模型;灌药假手术组(M3),手术灌药组(M4),每组10只;对照组10只.各组取脑组织切片免疫组化法测定Bcl-2基因表达并作比较分析. 结果 4组脑细胞均有Bcl-2基因表达,M4组比M2、M3组多(p<0.01,p<0.001)M2组比M3组多(p<0.001),M1组与M3组无差别,即急性脑缺血模型的Bcl-2基因表达出现最多.对照组Bcl-2基因表达无数为零. 结论①何首乌能通过增加Bcl-2基因的表达以提高脑细胞抗衰老的能力;②何首乌能通过增加脑细胞Bcl-2基因的表达提高缺血状态下脑细胞的保护作用.
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