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The HBV Genotype of Children Living in Guangzhou
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1674-1927.2002.01.003
- Key Word:
- 肝炎病毒;乙型;基因型
Abstract： 目的扩增HBV DNA S基因,建立HBV DNA的分型方法,研究广州地区小儿无症状乙肝病毒携带者(AsC)的基因型. 方法利用PCR-RFLP基因分型方法,扩增HBV DNA S基因,以限制性内切酶AvaII、MboI酶切分型. 结果 60份AsC血清样本,B型37份(62.7%),C型20份(33.3%),B、C混合型2份(3.3%),未分型1份(1.7%),经序列分析证明为C型,C型共35%. 结论 PCR-RELP分型方法经济实用,分型率达98.3%,广州地区小儿乙肝病毒的基因型以B型和C型为主,其中携带者中B型占优势.
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