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Expression of Vascular Endothelial Growth Factor and Detection of Microvessel in Human Brain Gliomas and its Clinical Significance
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1674-1927.2001.06.005
- Key Word:
- 血管内皮细胞生长因子;微血管数量;胶质瘤;免疫组织化学
Abstract： 目的探讨人脑胶质瘤中血管内皮细胞生长因子(VEGF)的表达与微血管数量(MVQ)和肿瘤良恶性程度的关系. 方法用免疫组织化学染色方法检测手术切除石蜡包埋的47例人脑胶质瘤(Ⅰ～Ⅱ级22例,Ⅲ级15例,Ⅳ级10 例)组织中的VEGF蛋白表达情况和MVQ数量,借助显微镜观察其阳性细胞数和阳性血管数; 另外,分别取脑外伤后开颅内减压术中切除的正常脑组织10例作为对照组. 结果①正常脑组织未检测到VEGF蛋白的表达,而MVQ数量少 ,表达强度弱;②各级别胶质瘤组织中均有VEGF和MVQ表达,主要表达于肿瘤细胞的胞浆和血管内皮细胞胞膜中;③3组胶质细胞瘤中VEGF的表达阳性率分别为22.2%、86.7%、90%、Ⅰ～Ⅱ级组与其它两组间差异有显著性意义(p<0.05;p<0.01),VEGF表达阳性组的MVQ平均值(46.8±12.4)明显高于阴性组MVQ值 (22.4±11.5)(p<0.01);④VEGF的表达与MVQ相关(r=0.75, p <0.001). 结论①VEGF在脑胶质瘤血管生成中起重要作用 ,能促进胶质瘤血管的形成;②VEGF和MVQ与胶质瘤良恶性程度明显相关,可作为脑胶质瘤病理诊断的补充指标;③VEGF可作为治疗脑胶质瘤的靶向,为胶质瘤的基因治疗提供指导方向.
- [1]J, Folkman.Tumor angiogenesis: therapeutic implications.[J].1971,285.
- [2]N, Weidner,J, Folkman,F, Pozza,etc.Tumor angiogenesis: a new significant and independent prognostic indicator in early-stage breast carcinoma.[J].1992,84.
- [3]Laura E. Benjamin,Eli Keshet.Conditional switching of vascular endothelial growth factor (VEGF) expression in tumors: Induction of endothelial cell shedding and regression of hemangioblastoma-like vessels by VEGF withdrawal[J].1997,94(16).
- [4]DiSalvo J,Bayne ML,Conn G,etc.Purification and characterization of a naturally occurring vascular endothelial growth factor.placenta growth factor heterodimer.[J].1995,270(13).
- [5]M. Moriyama,S. Kumagai,S. Kawashiri,etc.Immunohistochemical study of tumour angiogenesis in oral squamous cell carcinoma[J].1997,33(5).
- [6]Holash, J.,Maisonpierre, P.C.,Compton, D.,etc.Vessel Cooption, Regression, and Growth in Tumors Mediated by Angiopoietins and VEGF.[J].1999,284(5422).
- [7]K, Maeda,Y S, Chung,Y, Ogawa,etc.Prognostic value of vascular endothelial growth factor expression in gastric carcinoma.[J].1996,77(5).