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The Research of Aspartic Protease Inhibitors from Soy Protein Hydrolyzed by Proteases
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- Author:
- No author available
- Journal Title:
- Natural Product Research and Development
- Issue:
- 8
- DOI:
- 10.3969/j.issn.1001-6880.2012.08.018
- Key Word:
- 酶解;大豆蛋白;天冬氨酸蛋白酶;抑制剂
Abstract： 本文通过采用可控酶解的方式酶解大豆蛋白获得了一种源自大豆的天冬氨酸蛋白酶抑制剂SAPI( soy aspartic proteinase inhibitor).酶解液经过DEAE-52阴离子交换层析、Superdex Peptide 10/300 GL凝胶层析、SOURCE 15RPC反相层析分离后最终比抑制活力为254.2 IU/mg,纯化倍数为62.SAPI对胃蛋白酶(3000U)半数抑制浓度IC50为25.67 μg/mL,有良好的热稳定性,属于一种非竞争性抑制剂.
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