Abstract: Twelve compounds were isolated from the ethanol extract of Hypoxis aurea,and their structures were elucidated by physicochemical properties and spectroscopic analysis as quercetin-3-O-β-D-glucoside(1),kaemferol-3-O-β-D-glucoside(2),apigenin-5-O-β-D- glucopyranoside(3),α-spinasterol (4),2,6-dimethoxy-benzoic acid (5),1H-indole-3-carboxylic acid (6),( 2S,3R,4E,8E) -1 - (β-D-glucopyranosyloxy) -3 -hydroxy-2 [ ( ( R ) -2′ -hydroxyeicosanoly ) amino ] -9-methy-4,8-octadecadiene(7),n-dotriacontanol (8),14,15-eicosenicacid (9),lignoceric acid(10),β-sitoster-ol ( 11 ),daucosterol(12).All these compounds were found in this plant for the first time.