Abstract: Young stems of Acer ukurunduense Trautv. et Mey., Acer tegmentosum Maxim., and Acer komarovii Pojark. were used as explants and suitable medium compositions for axillary bud growing and rooting were screened through Uniform Design experiments. The results showed that MS (modified)+IAA0.10 mg·L~(-1)+GA_3l.l0 mg·L~(-1) was most suitable for Acer ukurunduense with a regeneration rate of 98%; MS (modified) + IAA0.40 mg·L~(-1)+IBA0.10 mg·L~(-1)+NAA0.07 mg·L~(-1)+GA3l.l0 mg·L~(-1) was most suitable for Acer tegmentosum with a regeneration rate of 96%; MS (modified)+IBA0.10 mg·L~(-1)+GA_3l.l0 mg·L~(-1)was most suitable for Acer komarovii with a regeneration rate of 94%. Stems each with one node were cut from regenerated shoots and cultured for propagation, and a 65-fold proliferation rate was achieved within 30~35 days.