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Effects of long-term intensive management on soil ammonia oxidizing archaea community under Phyllostachys praecox stands

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Author:: No author available

Journal Title:: Acta Ecologica Sinica

Issue:: 19

DOI:: 10.5846/stxb201108251240

Key Word:: 集约种植;雷竹林土壤;氨氧化古菌;典范对应分析

Abstract：应用荧光定量PCR以及PCR-DGGE技术研究了雷竹林长期集约种植过程中土壤氨氧化古菌种群数量及群落结构的演变趋势,并利用典范对应分析(CCA)方法研究影响土壤氨氧化古菌群落的主要环境因子.研究结果表明,水稻田改种雷竹后,土壤氨氧化古菌种群数量显著增加,在4a时达到最高,但在集约种植后快速下降,9a雷竹林土壤氨氧化古菌数量最低,随后逐渐稳定.雷竹林土壤氨氧化古菌种群主要为不可培养泉古菌,聚类分析结果表明集约种植前后氨氧化古菌群落结构存在明显差异,长期集约经营雷竹林土壤以适应较低pH值的物种为主要优势种群.CCA分析表明,集约种植时间较长的11a和15a林地群落结构较为类似,与7a和9a样地明显区分.土壤NO3--N与氨氧化古菌群落变化的相关性最强,说明氨氧化古菌在雷竹林土壤硝化过程中发挥着重要作用.土壤pH值及速效养分对土氨氧化古菌群落也具有较大影响,它们与NO3-N合计解释了59.7％的样本总变异,表明土壤酸化以及过量养分的积累对氨氧化古菌群落具有重要影响.

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