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Establishment of H reflex model in mice with minimal insult and measurement of nerve conduction velocity

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Author:: No author available

Journal Title:: Acta Physiologica Sinica

Issue:: 4

DOI:: No doi available

Key Word:: 肌电图;小鼠;H反射;动物模型;神经传导

Abstract：本文旨在建立小鼠后肢微创H反射模型,为临床脊髓损伤等相关疾病研究提供个简便易行的诱导H反射的实验方法.对浅麻醉昆明小鼠后肢进行微创手术:在下肢股骨大转子与尾根部连线中外1/3交点处切开皮肤,将一对细铜线分别穿经浅层肌肉并绑扎固定于坐骨神经附近组织,作为第一对刺激电极；在踝部以上跟腱内侧靠近胫神经处浅层肌肉穿入另对电极,作为第二对刺激电极；将细铜线穿过跖骨近端表面皮肤并绑扎固定作为记录电极；参考电极置于后肢拇趾根部足垫.把相应电极连接到肌电图诱发电位仪,记录M波和H波,并利用在下肢两不同刺激点所诱导的M波和H波潜伏期的差值测量神经传导速度.此模型H反射引出率在坐骨神经和胫神经处分别为92.73％和81.82％,H波潜伏期约为7～10 ms.测得运动神经传导速度(motor nerve conduction velocity,MNCV)和感觉神经传导速度(sensory nerve conductionvelocity,SNCV)分别为(25.84±4.70)和(31.45±7.31)m/s.这种在小鼠后肢建立的微创H反射模型简便易行,对小鼠损伤轻微,所获得的波形干扰小,非常适用于对H反射潜伏期的研究以及需要小鼠较长时间保持良好生理状态的实验项H.此模型也可用于感觉和运动神经的传导速度检测.

Reference

[1]刘磊,邵政一.神经再生素对糖尿病大鼠周围神经病变治疗作用研究[J].2007,(2).
[2]李光华,刘宏鹏,周旭,等.运动训练对坐骨神经损伤小鼠神经形态和功能恢复影响的研究[J].2010,(1).
[3]程茅薇,顾萍,马健,等.西格列汀对db/db糖尿病小鼠坐骨神经传导速度的影响[J].2011,(18).
[4]王佳,刘铜华.糖痹康对糖尿病周围神经病变大鼠坐骨神经传导速度的影响[J].2010,(3).
[5]Lukacova N,Marsala J,Wohlfahrt P,etc.The evidence for nitric oxide synthase immunopositivity in the monosynaptic Ia-motoneuron pathway of the dog.[J].2005,195(1).
[6]Knikou M.The H-reflex as a probe: pathways and pitfalls.[J].2008,171(1).
[7]Higashimori H,Carlsen RC,Whetzel TP.Early excision of a full-thickness burn prevents peripheral nerve conduction deficits in mice.[J].2006,117(1).
[8]Chen Y,Wang Y,Chen L,etc.H-reflex up-conditioning encourages recovery of EMG activity and H-reflexes after sciatic nerve transection and repair in rats.[J].2010,30(48).
[9]Deneri E,Ozkul A,Tataroglu C.Adductor T reflex abnormalities in patients with decreased patellar reflexes.[J].2009,40(2).
[10]陈恒胜,刘连生,廖维宏.大鼠脊髓损伤及继发损伤时脊髓诱发电位与运动诱发电位的变化[J].1995,(2).
[11]Adams MM,Hicks AL.Comparison of the effects of body-weight-supported treadmill training and tilt-table standing on spasticity in individuals with chronic spinal cord injury.[J].2011,34(5).
[12]Keller BU,Steffens H,Zschuntzsch J,etc.Fatigability of spinal reflex transmission in a mouse model (SOD1(G93A)) of amyotrophic lateral sclerosis.[J].2011,43(2).
[13]Carp JS,Tennissen AM,Wolpaw JR,etc.Diurnal H-reflex variation in mice.[J].2006,168(4).
[14]Carlsen RC,Stewart TA,Elias KA,etc.Peripheral neuropathy in transgenic diabetic mice: restoration of C-fiber function with human recombinant nerve growth factor.[J].1998,47(10).
[15]Emad MR,Gheisi AR.A complementary approach for evaluating S1-root in diabetic neuropathic patients.[J].2010,50(1).
[16]Lee HJ,Jakovcevski I,Radonjic N,etc.Better functional outcome of compression spinal cord injury in mice is associated with enhanced H-reflex responses.[J].2009,216(2).
[17]Fuchigami T,Kakinohana O,Hefferan MP,etc.Potent suppression of stretch reflex activity after systemic or spinal delivery of tizanidine in rats with spinal ischemia-induced chronic spastic paraplegia.[J].2011,194.
[18]Lagarce F,Richard J,Benoit JP,etc.Baclofen-loaded microspheres: preparation and efficacy testing in a new rabbit model.[J].2005,59(3).
[19]Mori F,Motoyama S,Tajima T,etc.Characteristics of H- and M-waves recorded from rat forelimbs.[J].2009,450(3).