Effects of carnitine on respiratory chain and metabolism of oxygen radical in mitochondria of liver after exhaustive running in training rat
- Author:
- No author available
- Journal Title:
- Acta Physiologica Sinica
- Issue:
- 4
- DOI:
- No doi available
- Key Word:
- 肉碱;肝脏;线粒体;电子传递链酶复合体;氧自由基
Abstract： 本研究旨在探讨肉碱对运动训练大鼠肝脏细胞线粒体电子传递链及氧自由基代谢的影响.将雄性 Wistar大鼠40只随机均分为4组(n=10):对照组、肉碱组、训练组和训练+肉碱组.训练组和训练+肉碱组进行递增负荷水平跑台运动训练,肉碱组和训练+肉碱组每日灌胃口服肉碱(300 mg/kg)次,每周6天,共处理6周.6周后备组大鼠均进行力竭运动,随后立刻取肝脏样本,用差速离心法、提取肝脏线粒体,分光光度法测定线粒体电子传递链酶复合体Ⅰ～Ⅳ (CⅠ～Ⅳ)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性及丙二醛(MDA)含量.结果显示,与对照组相比,肉碱组CⅣ活性显3著升高(P＜ 0.05),训练组CⅠ、CⅢ、CⅣ活性均显著升高(P＜ 0.05或P＜ 0.01),训练+肉碱组CⅠ～Ⅳ活性均显著升高(P＜ 0.05或P＜0 01)；与肉碱组和训练组相比,训练+肉碱组CⅠ和CⅣ活性均显著升高(P＜0.05或P＜0.01).与对照组相比,训练+肉碱组SOD活性显著升高(P＜ 0.01),肉碱组、训练组和训练+肉碱组GSH-Px活性均显著升高(P＜ 0.05或P＜ 0.01),训练组和训练+肉碱组MDA水平均显著降低(P＜0.05或P＜0.01):与肉碱组相比,训练+肉碱组GSH-Px活性显著升高(P＜0.01)；与肉碱组和训练组相比,训练+肉碱组SOD活性均显著升高(P＜ 0.05或P＜0.01),MDA水平均显著降低(P＜ 0.01或P＜ 0.05).以上结果提示,运动训练、及补充肉碱均可提高肝脏线粒体电子传递链功能及抗氧化能力,且运动训练与肉碱的作用具有协同效应.
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