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Conserved motifs in voltage sensing proteins

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Author:: No author available

Journal Title:: Acta Physiologica Sinica

Issue:: 4

DOI:: No doi available

Key Word:: 膜电位;电压感受蛋白;保守基序;电压门控型离子通道;电压依赖性磷酸激酶

Abstract：本文旨在研究电压感受蛋白(voltage sensing proteins,VSPs)的保守基序、分析其序列特点并组建其电压感受模型.在UniProt数据库中的检索结果显示,现有的VSPs主要为电压门控型离子通道及电压依赖性磷酸激酶,其共同特点是均含有一个4次跨膜区,分别称为S1～S4 (Segment 1-4).S1区主要负责其上膜与转运,S2～S4区是其感知膜电位变化的核心.profile-to-profile序列比对结果表明VSPs各跨膜区的保守基序非常相似,尤其是S4跨膜区均含基序[RK]-X(2)-R-X(2)-R-X(2)-[RK],其特点是带正电荷的精氨酸残基(R)与两个疏水性或不带电荷的氨基酸残基交替排列,这是VSPs感知膜电位变化的核心区域.同时,四个跨膜区内均含大量具有较高α-螺旋结构形成倾向性的疏水性氨基酸残基,这可能是VSPs(尤其是带有大量正电荷的S4区域)能在胞膜上稳定存在的主要原因.此外,S3区内带负电荷的天冬氨酸残基(D)的保守性对VSPs感知膜电位变化具有重要意义,天冬氨酸残基与S4区的静电作用还可增加S4区在膜上的稳定性.

Reference

[1]Isacoff EY,Tombola F,Pathak MM.How far will you go to sense voltage?[J].2005,48(5).
[2]Sang-Ah Seoh,Daniel Sigg,Diane M Papazian,etc.Voltage-Sensing Residues in the S2 and S4 Segments of the Shaker K+ Channel[J].1996,16(6).
[3]Guda C,Bourne PE,Guda P.Conserved motifs in voltage-sensing and pore-forming modules of voltage-gated ion channel proteins[J].2007,352(2).
[4]Jentsch TJ,Dedek K,Steinlein OK,etc.Myokymia and neonatal epilepsy caused by a mutation in the voltage sensor of the KCNQ2 K+ channel.[J].2001,98(21).
[5]Naibo Yang,Richard Horn.Evidence for voltage-dependent S4 movement in sodium channels[J].1995,15(1).
[6]Chahine M,Takami H,Sato C,etc.Role of arginine residues on the S4 segment of the Bacillus halodurans Na+ channel in voltage-sensing[J].2004,201(1).
[7]Okamura Y.Biodiversity of voltage sensor domain proteins.[J].2007,454(3).
[8]Isacoff EY,Tombola F,Pathak MM.Voltage-sensing arginines in a potassium channel permeate and occlude cation-selective pores.[J].2005,45(3).
[9]Naibo Yang,Alfred L George Jr.,Richard Horn.Molecular Basis of Charge Movement in Voltage-Gated Sodium Channels[J].1996,16(1).
[10]Iwasaki H,Okochi Y,Hossain MI,etc.Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish voltage-sensing phosphatases[J].2008,283(26).
[11]Bezanilla F.How membrane proteins sense voltage.[J].2008,9(4).
[12]Banerjee A,MacKinnon R.Inferred motions of the S3a helix during voltage-dependent K+ channel gating.[J].2008,381(3).
[13]Robert Robertson,Nicholas P. Davies,Dimitri M. Kullmann,etc.Dysfunction of the brain calcium channel Ca_v2.1 in absence epilepsy and episodic ataxia[J].2004,127(Pt.12).
[14]Sansom MSP,Sands ZA.How does a voltage sensor interact with a lipid bilayer? Simulations of a potassium channel domain[J].2007,15(2).
[15]Saier MH Jr*,Montal M,Kuan G,etc.Modular assembly of voltage-gated channel proteins: A sequence analysis and phylogenetic study[J].1999,1(2).
[16]Timothy L. Bailey,Michael E. Baker,Charles P. Elkan.An artificial intelligence approach to motif discovery in protein sequences: Application to steroid dehydrogenases[J].1997,62(1).
[17]Chanda B,Bezanilla F.A common pathway for charge transport through voltage-sensing domains.[J].2008,57(3).
[18]Luce S,Fontaine B,Carle T,etc.Gating defects of a novel Na+ channel mutant causing hypokalemic periodic paralysis[J].2006,348(2).
[19]Chouabe C.,Guicheney P.,Neyroud N.,etc.Properties of Kvlqt1 K+ Channel Mutations in Romano-Ward and Jervell and Lange-Nielsen Inherited Cardiac Arrhythmias[J].1997,16(17).
[20]Arhem P.Voltage sensing in ion channels: a 50-year-old mystery resolved?[J].2004,363(9416).