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Regulation of pyloric rhythm by IA and Ih in crayfish stomatogastric ganglion

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Author:: No author available

Journal Title:: Acta Physiologica Sinica

Issue:: 3

DOI:: No doi available

Key Word:: 中枢模式发生器;口胃神经节;幽门节律;4AP;ZD7288

Abstract：甲壳类动物的口胃神经节(stomatogastric ganglion,STG)由30个神经元组成,产生幽门节律,是研究中枢模式发生器(central pattern generator,CPG)的常用模型.幽门节律的维持与调控,不仅与STG神经元网络中单个神经元的特性有关,也与整个网络的连接及其性质相关.已有研究观察到STG网络中不同神经元上存在瞬时外向钾电流(transient potassium current,IA)与超极化激活阳离子电流(hyperpolarization-activated cation current,Ih),但这两种电流对整个STG网络节律的维持与调控作用仍不清楚.本实验采用鳌虾STG离体标本神经纤维记录,通过施加特异性阻断剂来研究IA和Ih对鳌虾STG幽门节律性放电的调节作用.结果显示:施加IA特异性阻断剂4AP(2 mmol/L)后,幽门节律周期变短(P＜0.01),PD (pyloric dilator)细胞放电时程占整个三相周期的比率增加(P＜ 0.01),LP (lateral pyloric)细胞放电时程不变(P＞0.05)但相位滞后,PY (pyloric)细胞放电时程占整个三相周期的比率减小(P＜0.05)且相位滞后,三相顺序没有改变;施加Ih特异性阻断剂ZD7288 (100 μmol/L)后,幽门节律周期变短(P＜0.01),PD细胞放电时程占整个三相周期的比率增加(P＜0.01),LP细胞放电时程占整个三相周期的比率增加(P＜ 0.01)且相位滞后,PY细胞放电时程占整个三相周期的比率减小(P＜0.01)且相位滞后,三相顺序没有改变.上述结果表明,IA和Ih对鳌虾STG幽门节律有重要的调节作用,进而影响到幽门活动的生理功能.

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