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Involvement of inhibition of nucleus GAPDH over-expression in erythropoietin's reduction of neuronal apoptosis induced by brain ischemia/reperfusion in rats
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- Author:
- No author available
- Journal Title:
- Acta Physiologica Sinica
- Issue:
- 3
- DOI:
- No doi available
- Key Word:
- 促红细胞生成素;GAPDH;脑缺血;神经元凋亡
Abstract： 本文旨在探讨重组人红细胞生成素(recombinant human erythropoietin,rhEPO)是否通过抑制甘油醛三磷酸脱氢酶(glyceraldehyde-3-phosphate dehydrogenase,GAPDH)核内过表达减少脑缺血再灌注大鼠神经元凋亡.大脑中动脉栓塞法制作脑缺血再灌注大鼠模型.48只Sprague-Dawley大鼠随机分为假手术组、生理盐水对照组和EPO处理组.TTC染色、尼氏染色及Hoechst-33258免疫荧光分别观察EPO对脑组织缺血和缺血半影区神经元凋亡的影响;Hoechst-33258和GAPDH免疫荧光双染观察EPO对GAPDH核内过表达的影响.结果显示,与生理盐水对照组相比,再灌注同时开始给予rhEPO(3 000 U/kg,3次/日,腹腔注射)显著抑制缺血再灌注引起的神经元GAPDH核内过表达,同时显著减少半影区神经元凋亡数目,减轻缺血性脑损伤.以上结果提示,rhEPO可能通过抑制GAPDH核内过表达减少脑缺血再灌注大鼠半影区神经元凋亡,为探讨EPO的神经保护机制提供了实验证据.
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