Lessening effect of hypoxia-preconditioned rat cerebrospinal fluid on oxygenglucose deprivation-induced injury of cultured hippocampai neurons in neonate rats and possible mechanism
- Author:
- No author available
- Journal Title:
- Acta Physiologica Sinica
- Issue:
- 6
- DOI:
- No doi available
- Key Word:
- 低氧预适应;脑脊液;海马神经元;Bcl-2/Bax
Abstract： 本文旨在观察低氧预适应(hypoxic preconditioning,HPC) Wistar大鼠脑脊液对新生鼠海马神经元氧糖剥夺(oxygen glucose deprivation,OGD)损伤的影响及机制.原代培养大鼠新生鼠(出生12 h)海马神经元,随机分为正常对照组、OGD组(OGD培养1.5 h)、正常脑脊液组(正常大鼠脑脊液培养1 d+OGD培养1.5 h)和HPC脑脊液组(HPC大鼠脑脊液培养1 d+OGD培养1.5 h),各组n=6.用含1 mmol/L Na2S2O4的无糖Earle's液培养大鼠新生鼠海马神经元1.5 h建立OGD模型.应用激光共聚焦显微镜和流式细胞术检测各组神经元凋亡情况,用免疫荧光染色法检测各组神经元Bcl-2/Bax表达水平.结果显示,正常对照组偶见凋亡早期细胞,OGD组见大量中晚期凋亡细胞；与OGD组和正常脑脊液组相比,HPC脑脊液组细胞凋亡显著减少(P＜ 0.01),细胞生存率提高(P＜0.01).OGD组和正常脑脊液组细胞Bcl-2蛋白荧光强度分别为0.040±0.003,0.457±0.044,而HPC脑脊液组细胞Bcl-2表达荧光强度增至1.923±0.078 (P＜ 0.01),且阳性细胞数量亦明显增加(P＜ 0.01); Bax表达则呈相反趋势,相对OGD组(1.548±0.068)和正常脑脊液组(1.072±0.219),HPC脑脊液组荧光强度(0.411±0.039)明显降低(P＜0.01),且阳性细胞数量亦明显减少(P＜0.01).以上结果提示,HPC脑脊液能减轻OGD损伤诱导的神经元凋亡,该神经保护效应与上调Bcl-2表达、下调Bax表达,提高Bcl-2/Bax有关.
- [1]崔秀玉,李凌,安仰原,等.低氧预处理小鼠脑及血液中糖原与乳酸含量的变化[J].2001,(4).
- [2]刘亮,吕国蔚.缺氧预适应小鼠脑匀浆去蛋白液对缺氧突触体膜的保护作用[J].2001,(4).
- [3]Bezin L,Jackson TS,Groc L,etc.Bax, Bcl-2, and cyclin expression and apoptosis in rat substantia nigra during development.[J].2001,306(3).
- [4]Liotta LA,Espina BH,Petricoin EF 3rd,etc.CSF proteome: a protein repository for potential biomarker identification.[J].2005,2(1).
- [5]Tjulkova E,Samoilov MO,Rybnikova E,etc.The preconditioning modified neuronal expression of apoptosis-related proteins of Bcl-2 superfamily following severe hypobaric hypoxia in rats.[J].2006,1089(1).
- [6]M H, Epstein,A M, Feldman,S W, Brusilow.Cerebrospinal fluid production: stimulation by cholera toxin.[J].1977,196.
- [7]Gidday JM.Cerebral preconditioning and ischaemic tolerance[J].2006,7(6).
- [8]Zappaterra MW,D'Ercole AJ,Walsh CA,etc.The cerebrospinal fluid provides a proliferative niche for neural progenitor cells.[J].2011,69(5).
- [9]Johanson C,Stopa E,Sharma H,etc.Traumatic brain injury and recovery mechanisms: peptide modulation of periventricular neurogenic regions by the choroid plexus-CSF nexus.[J].2011,118(1).
- [10]Hallenbeck JM,Ginis I,Chen Y.The protective effect of ceramide in immature rat brain hypoxia-ischemia involves up-regulation of bcl-2 and reduction of TUNEL-positive cells.[J].2001,21(1).
- [11]Vannucci SJ,Vannucci RC,Towfighi J.Hypoxic preconditioning and hypoxic-ischemic brain damage in the immature rat: pathologic and metabolic correlates.[J].1998,71(3).
- [12]Reed J. C..Bcl-2 and the regulation of programmed cell death[J].1994.