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Decreased neurotrophin-3 expression of intrafusal muscle fibers in rat soleus muscles under simulated weightlessness
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- No doi available
- Key Word:
- 失重;比目鱼肌;肌梭;神经营养因3
Abstract： 本研究旨在探讨模拟失重对大鼠比目鱼肌肌梭神经营养因子3(neurotrophin-3,NT-3)表达的影响.采用大鼠尾部悬吊法建立模拟失重动物模型,按体重配对原则随机将大鼠分为5组,即尾悬吊3 d组、7 d组、14 d组、21 d组和正常同步对照组.采用免疫组织化学ABC染色法及酶联免疫吸附实验法(ELISA)检测大鼠比目鱼肌肌梭NT-3的表达.结果显示,大鼠比目鱼肌梭外肌中未见到NT-3表达;正常对照组大鼠比目鱼肌肌梭中,核袋1和核袋2纤维NT-3旱现强阳性表达;模拟失重后,梭内肌纤维的NT-3免疫染色反应进行性降低;NT-3的ELISA定量检测结果显示,正常组、尾悬吊3 d组、7 d组、14 d组和21 d组大鼠比目鱼肌NT-3的含量分别为(14.23±1.65)、(14.11±1.53)、(13.09±1.47)、(12.45±1.51)和(9.85±1.52)pg/mg.统计比较显示,尾悬吊14 d后,大鼠比目鱼肌NT-3的含量较正常对照组明显减少(P<0.05);而尾悬吊21 d后,大鼠比目鱼肌NT-3的表达进一步减少(P<0.01).以上结果表明,模拟失重可致大鼠比目鱼肌肌梭NT-3的表达明显减少,并且随着模拟失重时间的延长,梭内肌NT-3的表达量进行性减少.提示微重力环境下本体感觉的适应性变化可能与肌梭NT-3的表达减少有关.
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