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Overexpression of β1-adrenoceptor can not protect rat cardiomyocytes from injury induced by isoprenaline
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- No doi available
- Key Word:
- β-肾上腺素受体;心肌细胞;收缩;异丙肾上腺素
Abstract： 本文旨在观察转染β1-肾上腺素受体(β1-adrenoceptor,β1-AR)基因对异丙肾上腺素(isoprenaline,ISO)损伤的大鼠心肌细胞收缩功能和存活的影响.采用胶原酶消化法分离培养原代大鼠心肌细胞,转染含β1-AR基因的腺病毒后,用ISO孵育24 h致心肌细胞损伤,采用Western blot检测心肌细胞β1-AR蛋白的含量,通过计数培养细胞中杆状细胞的百分率来检测心肌细胞存活率,用可视化边缘探测系统检测心肌细胞收缩功能变化.结果显示,与对照组相比,转染β1-AR基因的心肌细胞β1-AR蛋白含量明显增加(P＜0.01);转染β1-AR基因并用ISO损伤的心肌细胞内β1-AR蛋白含量子力学亦明显增加(P＜0.01).与对照组相比,转染β1-AR基因的心肌细胞存活率没有明显变化,ISO损伤的心肌细胞存活率明显下降(P＜0.01):而转染β1-AR基因并用ISO损伤的心肌细胞存活率与ISO组比进一步下降(P＜0.01).与对照组相比,转染β1-AR基因的心肌细胞收缩幅度显著增大(P＜0.05).ISO损伤的心肌细胞收缩幅度明显下降(P＜0.01);而转染β1-AR基因并用ISO损伤的心肌细胞的收缩幅度与ISO组比没有明显变化.以上结果提示,β1-AR的表达增加对ISO损伤的大鼠心肌细胞的保护作用不明显.
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