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Calpain mediates cardiac troponin Ⅰ degradation in tail-suspended rats
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 5
- DOI:
- No doi available
- Key Word:
- 钙蛋白酶;钙蛋白酶抑素;心肌肌钙蛋白抑制亚基;模拟失重;蛋白降解
Abstract： 本文旨在观察尾部悬吊模拟失重大鼠心肌钙蛋白酶(calpain)与钙蛋白酶抑素(calpastatin)表达的变化,以探讨心肌肌钙蛋白抑制业基(cardiac troponin Ⅰ,cTnⅠ)降解的可能机制.采用尾部悬吊模拟失重大鼠模型,Western blotting技术观测心肌calpain-1、calpain-2与calpastatin的表达;PD150606抑制calpain活性,分析cTnⅠ降解程度的变化.结果显示:与同步对照组相比,悬吊2周与4周组大鼠心肌calpastatin表达呈显著性降低(P＜0.05),calpain-1表达未改变,calpain-2表达略有降低:但是,心肌calpain-1/calpastatin及calpain-2/calpastatin的比值在悬吊2周与4周组明显增高(P＜0.05,P＜0.01).悬吊4周组cTnⅠ降解显著高于对照组(P＜0.01);然而,用calpain非特异件抑制剂PD150606处理后,对照组及悬吊组cTnⅠ的降解均被显著抑制(P＜0.01).这些结果提示模拟失重大鼠心肌calpain活性增高可能增加cTnⅠ的降解.
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