Olfactory ensheathing cells promote the survival of newborn rat spiral ganglion cells in vitro
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 2
- DOI:
- No doi available
- Key Word:
- 嗅鞘细胞;螺旋神经节细胞;培养;脑源性神经营养因子;存活
Abstract： 本文旨在探索离体实验中的嗅鞘细胞(olfactory ensheathing cells,OECs)有无促进耳蜗听觉传入神经元--螺旋神经节细胞(spiral ganglion cells,SGCs)存活作用及可能机制.取成年大鼠嗅球和新生大鼠蜗轴组织块进行OECs与SGCs的培养,采用差速贴壁法纯化培养OECs.实验分OECs与SGCs共培养组和SGCs单独培养组.倒置相差显微镜下观察OECs和SGCs生长状态,神经营养因子受体p75免疫组织化学法鉴定OECs,神经元特异性标志物βⅢ-tubulin标记SGCs.为了研究OECs与SGCs共培养体系中,前者促进后者存活的可能机制,共培养组中分别加入脑源性神经生长因子(BDNF,500pg/mL)和BDNF抗体(IgY型,50 μg/mL),对照组为未加任何处理的共培养组,然后检查各培养组中SGCs存活数量和存活时间.结果显示,OECs贴壁培养7 d后形成一细胞单层,在OECs与SGCs共培养体系中,SGCs在OECs形成的细胞单层的表面生长,并伸出长突起,呈现典型的双极神经元形态:在培养的前6天内,随着培养时间的增加,两组中的SGCs都较接种前减少,但共培养组中SGCs存活数量明显高于SGCs单独培养组(P<0.01);单独培养组的SGCs数量在培养的第6天出现人幅度减少,在培养的第9天几乎没有生长;共培养组的SGCs数量未见明显变化(P>0.05);共培养中加入BDNF对OECs促进SGCs存活无明显影响,而加入BDNF抗体(IgY)后存活的SGCs减少(P<0.01).本研究结果提示,OECs与SGCs共培养能够促进新生大鼠SGCs存活和突起生长,延长存活时间,OECs分泌BDNF可能是促进SGCs存活的机制之一.
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