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Nordihydroguaiaretic acid partially inhibits inflammatory responses after focal cerebral ischemia in rats

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Author:: No author available

Journal Title:: ACTA PHYSIOLOGICA SINICA

Issue:: 2

DOI:: No doi available

Key Word:: 去甲二氢愈创木酸;脑缺血;中性粒细胞;巨噬细胞;小胶质细胞;5-脂氧酶

Abstract：本实验旨在观察去甲二氢愈创木酸(nordihydroguaiaretic acid,NDGA)对大鼠局灶性脑缺血后炎症细胞聚集的作用及其机制.在大鼠大脑中动脉阻塞30 min后进行再灌注72 h,在再灌注30 min,2、24、48 h时分别腹腔注射一次NDGA(5、10 mg/kg).再灌注72 h后检测脑损伤、内源性IgG渗出、中性粒细胞和巨噬细胞/小胶质细胞聚集、细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)mRNA和蛋白表达,并在再灌注3 h后检测脑内5-脂氧酶(5-lipoxygenase,5LOX)的催化产物白烯B4(leukotriene B4,LTB4)和半胱氨酰白三烯(cysteinyl leukotrienes,CysLTs)含量.结果显示:NDGA能显著改善脑损伤,减少内源性IgG渗出、中性粒细胞浸润、ICAM-1 mRNA和蛋白表达,同时降低脑内LTB4和CysLTs含量,但对巨噬细胞/小胶质细胞聚集没有影响.上述结果提示,NDGA对脑缺血亚急性期炎症反应的抑制主要表现为减少中性粒细胞浸润,机制可能与抑制5-LOX激活有关.

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