The expression of proto-oncogene c-erbB_2 and its role in the initiation of primordial follicle growth in rat ovary

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Author:
XU Liang-Quan(Department of Physiology Reproduction, Nanchang University Medical College, Nanchang 330006, China)
XU Ai-Xia(Department of Physiology Reproduction, Nanchang University Medical College, Nanchang 330006, China)
HUANG Jian(Department of Physiology Reproduction, Nanchang University Medical College, Nanchang 330006, China)
CHEN Wei-Yun(Department of Physiology Reproduction, Nanchang University Medical College, Nanchang 330006, China)
ZHENG Yue-Hui(Department of Physiology Reproduction, Nanchang University Medical College, Nanchang 330006, China)
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
Volume 61, Issue 05, 2009
DOI:
Key Word:
ovary; primordial follicle; c-erbB_2; epidermal growth factor; RNAi

Abstract: Little is known about the factors that control the initiation of growth of primordial follicles. The objective of the present study was to investigate the effect of c-erbB_2 on the onset of primordial follicle development, and whether c-erbB_2 mediates the effect of epidermal growth factor (EGF) in this process. We synthesized three pairs of siRNAs targeting the c-erbB_2 mRNA and transferred them into the newborn rat ovary cultured in vitro with Metafectene. After siRNAs transfection, the efficiency of siRNAs was tested by examining c-erbB_2 mRNA and protein levels. The level of c-erbB_2 mRNA was reduced by 49.6%, 46.7% and 82.6% respectively after transfecting siRNA1, siRNA2 and siRNA3, and the level of ErbB_2 protein also reduced remarkably after siRNA3 transfection. c-erbB_2 siRNA3 significantly inhibited the primordial follicle initiation and development; EGF augmented primordial follicles formation, but the effect was abolished by c-erbB_2 siRNA3. All of these results suggest that c-erbB_2 plays an important role in primordial follicle development and folliculogenesis initiation, and mediates the effect of EGF on primordial follicle development.

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