The expression of proto-oncogene c-erbB_2 and its role in the initiation of primordial follicle growth in rat ovary

( views:, downloads: )
XU Liang-Quan(Department of Physiology Reproduction, Nanchang University Medical College, Nanchang 330006, China)
XU Ai-Xia(Department of Physiology Reproduction, Nanchang University Medical College, Nanchang 330006, China)
HUANG Jian(Department of Physiology Reproduction, Nanchang University Medical College, Nanchang 330006, China)
CHEN Wei-Yun(Department of Physiology Reproduction, Nanchang University Medical College, Nanchang 330006, China)
ZHENG Yue-Hui(Department of Physiology Reproduction, Nanchang University Medical College, Nanchang 330006, China)
Journal Title:
Volume 61, Issue 05, 2009
Key Word:
ovary; primordial follicle; c-erbB_2; epidermal growth factor; RNAi

Abstract: Little is known about the factors that control the initiation of growth of primordial follicles. The objective of the present study was to investigate the effect of c-erbB_2 on the onset of primordial follicle development, and whether c-erbB_2 mediates the effect of epidermal growth factor (EGF) in this process. We synthesized three pairs of siRNAs targeting the c-erbB_2 mRNA and transferred them into the newborn rat ovary cultured in vitro with Metafectene. After siRNAs transfection, the efficiency of siRNAs was tested by examining c-erbB_2 mRNA and protein levels. The level of c-erbB_2 mRNA was reduced by 49.6%, 46.7% and 82.6% respectively after transfecting siRNA1, siRNA2 and siRNA3, and the level of ErbB_2 protein also reduced remarkably after siRNA3 transfection. c-erbB_2 siRNA3 significantly inhibited the primordial follicle initiation and development; EGF augmented primordial follicles formation, but the effect was abolished by c-erbB_2 siRNA3. All of these results suggest that c-erbB_2 plays an important role in primordial follicle development and folliculogenesis initiation, and mediates the effect of EGF on primordial follicle development.

  • [1]Hoyer PE,Byskov AG,Mollgard K.Stem cell factor and cKit in human primordial germ cells and fetal ovaries.Mol Cell Endocrinol 2005; 234:1-10.
  • [2]Nilsson EE,Skinner MK.Kit ligand and basic fibroblast growth factor interactions in the induction of ovarian primordial to primary follicle transition.Mol Cell Endocrinol 2004; 214(1-2):19-25.
  • [3]Kezele P,Nilsson EE,Skinner MK.Keratinocyte growth factor acts as a mesenchymal factor that promotes ovarian primordial to primary follicle transition.Biol Reprod 2005; 73:967-973.
  • [4]Nilsson EE,Detzel C,Skinner MK.Platelet-derived growth factor modulates the primordial to primary follicle transition.Reproduction 2006; 131:1007-1015.
  • [5]Knight PG,Gliste C.TGF-β superfamily members and ovarian follicle development.Reproduction 2006; 132:191-206.
  • [6]Kezele PR,Nils son EE,Skinner MK.Insulin but not insulinlike growth factor-I promotes the primordial to primary follicle transiton.Mol Cell Endocrinol 2002; 192(1-2):37-43.
  • [7]Kevenaar ME,Meerasahib MF,Kramer P,van de Lang-Born BM,de Jong FH,Groome NP,Themmen AP,Visser JA.Serum anti-mullerian hormone levels reflect the size of the primordial follicle pool in mice.Endocrinology 2006; 147:3228-3234.
  • [8]Matzuk MM,Burns KH,Viveiros MM,Eppig JJ.Intercellular communication in the mammalian ovary:oocytes carry the conversation.Science 2002; 296:2178-2180.
  • [9]Brankin V,Mitchell MR,Webb B,Hunter MG.Paracdne effects of oocyte secreted factors and stem cell factor on porcine granulosa and theca cells in vitro.Reprod Biol Endocrinol 2003; 1:55.
  • [10]Thomas FH,Vanderhyden BC.Oocyte-granulosa cell interactions during mouse follicular development:regulation of kit ligand expression and its role in oocyte growth.Reprod Biol Endocrinol 2006; 4:19.
  • [11]Wang HB (王海滨),Li ML,Xia GL,Lu ZX,Guo Y,Xie HR.Effects of epidermal growth factor on the follicle development and steroidogenesis of mouse fetal ovary in vitro.J China Agric Univ (中国农业大学学报) 2001; 6(1):126(Chinese,English abstract).
  • [12]Liu HZ,Xu FH,Liu YX.Effects of epidermal growth factor on initiation of primordial follicle growth of newborn mouse.Sci China C Life Sci 2000; 30(5):535-543.
  • [13]Eppig JJ,O'Brien MJ.Development in vitro of mouse oocytes from primordial follicles.Biol Reprod 1996; 54(1):197-207.
  • [14]Jin X,Han CS,Zhang XS,Yuan JX,Hu ZY,Liu YX.Signal transduction of stem cell factor in primordial follicles development promotion.Mol Cell Endocrinol 2005; 229(1-2):3-10.
  • [15]Wang C,Roy SK.Expression of growth differentiation factor9 in the oocytes is essential for the development of primordial follicles in the hamster ovary.Endocrinology 2006; 147(4):1725-1734.
  • [16]Zheng YH (郑月慧),Zheng LP,Li F,Wu L,Dai YC.c-erbB_2 and c-myb induce oocyte maturation via activation of mitogenactivated protein kinase and maturation promoting factor.Acta Physiol Sin (生理学报) 2008; 60(1):97-104 (Chinese,English abstract).
  • [17]Thomas FH,Ethier JF,Shimasaki S,Vanderhyden BC.Follicle stimulating hormone regulates oocyte growth by modulation of expression of oocyte and granulosa cell factors.Endocrinology 2005; 146:941-949.
  • [18]Zheng P,Dean J.Oocyte-specific genes affect folliculogenesis,fertilization,and early development.Semin Reprod Med 2007; 25:243-251.
  • [19]Paradis F,Vigneault C,Robert C,Sirard MA.RNA interference as a tool to study gene function in bovine oocytes.Mol Reprod Dev 2005; 70:111-121.
  • [20]Tang F,Kaneda M,O'Carroll D,Hajkova P,Barton SC,Sun YA,Lee C,Tarakhovsky A,Lao K,Surani MA.Maternal microRNAs are essential for mouse zygotic development.Genes Dev 2007; 21:644-648.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615