The expression of proto-oncogene c-erbB_2 and its role in the initiation of primordial follicle growth in rat ovary
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 5
- DOI:
- 10.3321/j.issn:0371-0874.2009.05.004
- Key Word:
- 卵巢;原始卵泡;c-erbB_2;表皮生长因子;RNA干扰;ovary;primordial follicle;c-erbB_2;epidermal growth factor;RNAi
Abstract： Little is known about the factors that control the initiation of growth of primordial follicles. The objective of the present study was to investigate the effect of c-erbB_2 on the onset of primordial follicle development, and whether c-erbB_2 mediates the effect of epidermal growth factor (EGF) in this process. We synthesized three pairs of siRNAs targeting the c-erbB_2 mRNA and transferred them into the newborn rat ovary cultured in vitro with Metafectene. After siRNAs transfection, the efficiency of siRNAs was tested by examining c-erbB_2 mRNA and protein levels. The level of c-erbB_2 mRNA was reduced by 49.6%, 46.7% and 82.6% respectively after transfecting siRNA1, siRNA2 and siRNA3, and the level of ErbB_2 protein also reduced remarkably after siRNA3 transfection. c-erbB_2 siRNA3 significantly inhibited the primordial follicle initiation and development; EGF augmented primordial follicles formation, but the effect was abolished by c-erbB_2 siRNA3. All of these results suggest that c-erbB_2 plays an important role in primordial follicle development and folliculogenesis initiation, and mediates the effect of EGF on primordial follicle development.
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