Polydatin attenuates ischemia/reperfusion-induced apoptosis in myocardium of the rat

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Author:
ZHANG Li-Ping(Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China)
MA Hui-Jie(Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China)
BU Hui-Min(Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China)
WANG Mei-Ling(Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China)
LI Qian(Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China)
QI Zhao(Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China)
ZHANG Yi(Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China)
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
Volume 61, Issue 04, 2009
DOI:
Key Word:
polydatin;apoptosis;ischemia/reperfusion;heart;rat

Abstract: The aim of the present study was to investigate the effect of polydatin on apoptosis induced by iscbemia/reperfusion (I/R) in rat myocardium and to explore the underlying mechanism. Adult male Spragne-Dawley (SD) rats were randomly divided into control, I/R and polydatin (50 μmol/L) groups. On the Langendorff apparatus, isolated rat heart was subjected to 30-mln global ischemia followed by 60-min reperfusion. TUNEL labeling and flow cytometric techniques were used for the measurement of apoptosis and the expression of Bcl-2 and Bax protein in cardiomyocytes of rat. The results showed: (1) Compared with those in the control group, the number of TUNEL-positive cells and apoptosis rate were increased in I/R group; (2) Compared with that in the I/R group, the number of TUNEL-positive cells was significantly decreased in the polydatin group [(18.1±4.0)% vs (35.1±5.4)%, P<0.01]; (3) Apoptosis rate assayed by flow cytometry in I/R group was significantly higher than that in polydatin group [(15.43±4.55)% vs (8.66±3.18)%, P<0.01]; (4) Expression level of Bax protein was higher in I/R group than that in polydatin group (P<0.05), while the level of Bcl-2 protein and Bcl-2/Bax ratio were higher in polydatin group than those in I/R group (P<0.05, P<0.01), respectively. The results obtained suggest that polydatin exerts an inhibitory effect on I/R-induced apoptosis through increasing Bcl-2 protein expression and decreasing Bax protein expression in myocardium of the rat.

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