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Inhalation of aminoguanidine prevents the up-regulation of connective tissue growth factor in fibrotic lungs of rats
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0371-0874.2009.04.009
- Key Word:
- 氨基胍;诱导型一氧化氮合酶;结缔组织生长因子;博莱霉素;纤维化;肺;大鼠
Abstract： 本研究观察了雾化吸入氨基胍(aminoguanidine,AG)对博莱霉素(bleomycin,BLM)所致肺纤维化大鼠肺内结缔组织生长因子(connective tissue growth factor,CTGF)上调的影响.大鼠气管内一次性滴注BLM复制肺纤维化动物模型,以气管内滴注等容积的生理盐水(normal saline,NS)作对照.在气管滴注BLM后第1天至第30天期间,给大鼠雾化吸入AG(2 mmol/L、10 mmol/L或50 mmol/L,5 min/次,一天两次),以雾化吸入NS作对照.采用硝酸还原法、氯胺-T法、Masson染色法、Western blot和RT-PCR分别检测出肺血浆NO2-/NO3-含量(代表NO含量)、肺羟脯氨酸含量、肺胶原纤维含量以及肺CTGF蛋白和mRNA含量.结果显示:气管滴注BLM后第14天大鼠出肺血浆中NO2-/NO3-含量、气管滴注BLM后第30天大鼠的肺羟脯氨酸含量、肺胶原纤维含量、肺CTGF蛋白和mRNA水平均高于相应的对照组大鼠(均P<0.01);上述指标均在雾化吸入10 mmol/L和50 mmol/L AG后有所改善(出肺血浆中NO2-/NO3-含量P<0.01,其余均P<0.05).以上结果表明,AG能有效地防止肺纤维化大鼠肺内CTGF的上调,这可能是其防止肺纤维化形成的作用机制之一.
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