Slow rise of intracellular Ca2+ concentration in rat primary sensory neurons triggered by loureirin B

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Author:
YANG Yi-Ning(Laboratory of Neuropharmacology and Neurotoxicology, Shanghai University, Shanghai 200444, China)
CHEN Jue-Xu(Laboratory of Neuropharmacology and Neurotoxicology, Shanghai University, Shanghai 200444, China)
PANG Xue-Yan(Laboratory of Neuropharmacology and Neurotoxicology, Shanghai University, Shanghai 200444, China)
TERAKAWA Susumu(Photonmedical Research Center, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan)
CHEN Xu(Laboratory of Neuropharmacology and Neurotoxicology, Shanghai University, Shanghai 200444, China)
JI Yong-Hua(Laboratory of Neuropharmacology and Neurotoxicology, Shanghai University, Shanghai 200444, China)
YONG Ke-Lan(Laboratory of Neuropharmacology and Neurotoxicology, Shanghai University, Shanghai 200444, China)
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
Volume 61, Issue 02, 2009
DOI:
Key Word:
loureirin B;dorsal root ganglia neurons;[Ca2+]i;caffeine;Fura-2

Abstract: In the present study, the intracellular free calcium concentration ([Ca2+]i) in acutely isolated rat dorsal root ganglia (DRG) neurons modulated by loureirin B, an active component of "dragon' s blood" which is a kind of Chinese herbal medicine, was determined by the means of Fura-2 based microfluorimetry. It was found that ioureirin B could evoke the elevation of [Ca2+]i in a dose-dependent manner. However, the elevation of [Ca2+]i evoked in the calcium free solution was much smaller than that in the standard external cell solution, suggesting that most change of [Ca2+]i was generated by the influx of extracellular Ca2+, not by the activities of intracellular organelles like Ca2+ stores and mitochondria. In addition, the mixture of loureirin B and caffeine also induced [Ca2+]i rise, but the peak of [Ca2+]i rise induced by the mixture was significantly lower than that by caffeine alone, which means the triggering pathway and the targets of caffeine are probably involved in loureirin B-induced [Ca2+]i rise. Moreover, compared to the transients induced by caffeine, KCI and capsaicin, the loureirin B-induced [Ca2+]i rise is much slower and more stable. These results indicate that the capability of loureirin B of inducing the [Ca2+]i rise is solid and unique.

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