Simulated microgravity-induced oxidative stress in different areas of rat brain
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0371-0874.2009.02.002
- Key Word:
- 微重力;脑;氧化应激
Abstract： 本研究旨在观察不同时程模拟微重力效应下大鼠脑组织氧化应激的变化,为揭示模拟微重力效应下神经系统氧化应激的发生机制和开发相应的药物防护提供理论基础.将40只成年雄性Sprague-Dawley(SD)大鼠按体重配对原则随机分为对照组及不同时间(分别处理7,14,21,28 d)尾悬吊模拟微重力效应组,应用Griess法、硫代巴比妥酸反应物法(thiobarbituric acid reactive substance assay,TBARS)、ELISA法和铁离子还原法(ferric reducing ability of plasma,FRAP)分别检测大鼠小脑、大脑皮层和海马组织内的活性氮(reactive nitrogen species,RNS)、丙二醛(malondiaidehyde,MDA)、硝基酪氨酸(nitrotyrosine,NT)和总抗氧化能力(total antioxidant capacity,TAC).结果显示,与对照组相比:(1)小脑组织在尾悬吊7 d后NT含量明显升高,之后降低,28 d再次升高;14 d后MDA含量明显增加;21 d后RNS含量显著升高,TAC显著降低;(2)大脑皮层在尾悬吊14 d后NT含量显著升高;21 d后MDA含量明显升高,TAC明显降低;(3)海马组织在尾悬吊7 d后RNS含量明显升高,之后降低,28 d后再次升高;21 d后MDA含量明显升高;28 d后NT含量显著上升;7 d后TAC明显升高,持续至14 d,然后恢复.上述结果表明,模拟微重力效应使得大鼠脑组织发生了氧化应激,不同部位脑组织对氧化应激的反应程度不同;在对不同时程模拟微重力效应的响应过程中,大鼠脑组织呈现了从适应性反应到不可逆性损伤的变化历程.
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