Effects of acute cooling/rewarming on membrane potential and K+ currents in rat ventricular myocytes

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Author:
LI Long-Tian(Liaoning Key Laboratory of Biophysics,Institute of Basic Medical Sciences, Medical College, Dalian University, Dalian 116622, China;Department of Physiology, Xinxiang Medical College, Xinxiang 453003, China)
ZHANG Li-Bin(Liaoning Key Laboratory of Biophysics,Institute of Basic Medical Sciences, Medical College, Dalian University, Dalian 116622, China;Department of Physiology, Xinxiang Medical College, Xinxiang 453003, China)
SI Yan-Li(Department of Physiology, Xinxiang Medical College, Xinxiang 453003, China)
XIAO Fu-Cheng(Liaoning Key Laboratory of Biophysics,Institute of Basic Medical Sciences, Medical College, Dalian University, Dalian 116622, China)
LI Da(Liaoning Key Laboratory of Biophysics,Institute of Basic Medical Sciences, Medical College, Dalian University, Dalian 116622, China)
GAO Shan(Liaoning Key Laboratory of Biophysics,Institute of Basic Medical Sciences, Medical College, Dalian University, Dalian 116622, China)
LI Dong-Liang(Department of Physiology, Xinxiang Medical College, Xinxiang 453003, China)
ZHOU Shi-Sheng(Liaoning Key Laboratory of Biophysics,Institute of Basic Medical Sciences, Medical College, Dalian University, Dalian 116622, China)
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
Volume 60, Issue 03, 2008
DOI:
Key Word:
hypothermia;rewarming;potassium channels;membrane potentials;myocardium

Abstract: The effects of acute cooling/rewarming on cardiac K+ currents and membrane potential were investigated. Membrane potential and current were assessed with whole-cell patch-clamp technique in current-and voltage-clamp modes. When the temperature of bath solution was decreased from 25℃ to 4℃, the transient outward current (Ito) was completely abolished, the sustained outward K+ current (Iss) at +60mV and the inward rectifier K+ current (IK1) at-120mV were depressed by (48.5±14.1)% and (35.7±18.2)%, respectively, and the membrane potential became more positive. After the temperature of bath solution was raised from 4℃ to 36℃, the membrane potential exhibited a transient hyperpolarization and then was maintained at a stable level. In some myocytes (36 out of 58), activation of the ATP-sensitive K+ (KATP) channels after rewarming was observed. The rewarming-induced change in the membrane potential was inhibited by the Na+/K+-ATPase inhibitor ouabain (100μmol/L), and the rewarming-elicited activation of KATP channels was inhibited by the protein kinase A inhibitor H-89 (100μmol/L). Moreover, decrease of the temperature from 25℃ to 4℃ did not induce any significant change in cell volume when the cell membrane potential was clamped at O mV. However, significant ceil shrinkage with spots was observed soon after rewarming-induced activation of KATP channels. These data demonstrate that acute cooling/rewarming has a profound influence on the membrane potential and K+ currents of ventricular myocytes, and suggest that activation of KATP channels may play a role in cardiac cooling/rewarming injury.

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