Electrophysiological effects of hydrogen sulfide on pacemaker cells in sinoatrial nodes of rabbits

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Author:
XU Meng(Department of Physiology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China)
WU Yu-Ming(Department of Physiology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China)
LI Qian(Department of Physiology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China)
WANG Xin(Department of Physiology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China)
HE Rui-Rong(Department of Physiology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China)
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
Volume 60, Issue 02, 2008
DOI:
Key Word:
electrophysiology; hydrogen sulfide; action potential; sinoatrial node

Abstract: The cardiac electrophysiological effects of hydrogen sulfide (H2S) on pacemaker cells in sinoatrial (SA) nodes of rabbits wereexamined using intracellular microelectrode technique. The results obtained were as follows: (1) The velocity of diastolic (phase 4)depolarization (VDD) and rate of pacemaker firing (RPF) in normal pacemaker ceils in SA nodes were decreased by NariS (H2S donor)(50, 100, 200 μmol/L) in a concentration-dependent manner; (2) ATP-sensitive K+(KATP) channel blocker glybenclamide (Gli, 20 μmol/L) blocked the effect of NariS (100 μmol/L) on pacemaker cells; (3) Pretreatment with CsC1 (2 mmol/L), a blocker of pacemaker current(If), did not affect the effect of Naris (100 μmol/L) on SA node pacemaker cells; (4) DL-propargylglycine (PPG, 200 μmol/L), aninhibitor of cystathionine γ-lyase (CSE), did not affect the parameters of action potentials in pacemaker cells in SA nodes. All theseresults suggest that H2S exerts a negative chronotropic action on pacemaker cells in SA nodes of rabbits. These effects are likely due toan increase in potassium efflux through opening KATP channels; Ifis unlikely to play a major role in these effects. In our study, there wasno evidence for the generation of endogenous H2S by CSE in SA node pacemaker cells.

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