Ginkgolide B inhibits carotid sinus baroreflex in anesthetized male rats

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Author:
WANG Chun-Yan(Department of Physiology,Institute of Basic Medicine,Hebei Medical University,Shifiazhuang050017,China)
WU Yu-Ming(Department of Physiology,Institute of Basic Medicine,Hebei Medical University,Shifiazhuang050017,China)
XIAO Lin(Department of Physiology,Institute of Basic Medicine,Hebei Medical University,Shifiazhuang050017,China)
XUE Hong-Mei(Department of Physiology,Institute of Basic Medicine,Hebei Medical University,Shifiazhuang050017,China)
WANG Ru(Department of Physiology,Institute of Basic Medicine,Hebei Medical University,Shifiazhuang050017,China)
WANG Fu-Wei(Department of Physiology,Institute of Basic Medicine,Hebei Medical University,Shifiazhuang050017,China)
HE Rui-Rong(Department of Physiology,Institute of Basic Medicine,Hebei Medical University,Shifiazhuang050017,China)
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
Volume 60, Issue 01, 2008
DOI:
Key Word:
ginkgolide B;carotid sinus;baroreflex;tetraethylammonium

Abstract: The effects of ginkgolide B on the carotid sinus baroreflex(CSB)were studied in the perfused isolated carotid sinus of 30 anesthetized Sprague-Dawley male rats.The results were as follows.(1)By perfusing with ginkgolide B(0.1,1,10 μmol/L),the functional curve of the baroreflex was shifted to the right and upward.There was a marked decrease in peak slope(PS)and reflex decrease(RD)in mean arterial pressure(P<0.01),while the threshold pressure(TP),equilibrium pressure(EP)and saturation pressure (SP)were significantly increased(P<0.05,P<0.01).Among the functional parameters of CSB,the changes in PS,RD,TP,EP and SP were dose-dependent.(2)Pretreatment with Bay K8644(500 nmol/L),an agonist of L-type calcium channel,completely eliminated the effects of ginkgolide B(1 μmol/L)on the CSB.(3)Pretreatment with tetraethylammonium(TEA,1 mmol/L),an inhibitor of potassium channel,completely abolished the above effects of ginkgolide B(1 Iμmol/L)on the CSB.These results suggest that ginkgolide B inhibits the CSB in anesthetized rats,which iS mediated by decreased calcium influx and increased potassium efflux in baroreceptor nerve endings.

  • [1]Briskin DP.Medicinal plants and phytomedicines.Linking plant biochemistry and physiology to human health.Plant Physiol 2000:124(2):507-514.
  • [2]Kimbel KH.Ginkgo biloba.Lancet 1992;340(8828):1136-1139.
  • [3]Montrucchio G,Alloatti G,Canmssi G.Role of platelet-activating factor in cardiovascular pathophysiology.Physiol Rev 2000;80(4):1669-1699.
  • [4]Wang SJ,Chen HH.Chnkgolide B,a constituent of Ginkgo biloba,facilitates glutamate exocytosis from rat hippocampal nerve terminals.Eur J Pharmacol 2005;514(2-3):141-149.
  • [5]Debek W,Chyczewski L,Makarewicz M.Platelet-activating factor receptor-antagonist(BN 52021)stabilizes the oxidativeantioxidative balance and attenuates the morphological changes in the gastrointestinaltractinexperimentalhcmonlmgic shock.Exp Toxicol Pathol 1998;50(1):19-25.
  • [6]Sneddon AA,McLeod E,Wahle KW,Arthur JR.Cytokineinduced monocyte adhesion to endothelial cells involves platelet-activating factor:suppression by conjugated linoleic acid.Biochim Biophys Aeta 2006;1761(7):793-801.
  • [7]Flores NA,Goulielmos NV,Seghatchian MJ,Sheridan DJ.Myocardial ischaemia induces platelet activation with adverse electrophysiological and arrhythmogenic effects.Cardiovasc Res 1994;28(11):1662-1671.
  • [8]Valli G,Giardina EG.Benefits,adverse effects and drug interactions of herbal therapies with cardiovascular effects.J Am Coil Cardiol2002;39(7):1083-1095.
  • [9]Satoh H,Nishida S.Electropharmacological actions of Ginkgo biloba extract on vascular smooth and heart muscles.Clin Chim Acta 2004;342(1-2):13-22.
  • [10]YangLZ(仰礼真),Zhang Y,Li JX,Zhu YC,Zlang ZX,Li Y,Zhou ZN.Effects of BN-52021 on AP and L-Vype calcium channel in guinea pig's vcntricular myocytes.Chin Pharmaco BuU(中国药理学通报)2002;16(2):195-198(Chinese,EngUsh abstract).
  • [11]Zhang ZX,Qi XY,Xu YQ.Effect of ginkgolide B onL-type calcium current and cytosolic[Ca2+]in guinea pig ischemic venrricular myocytes.Acta Physiol Sin(生理学报)2003;55(1):24-28.
  • [12]Qi XY,Zhang ZX,Xu YQ.Effects of Ginkgolide B on action potential and calcium,potassium current in guinea pig ventricular myocytes.Acta Phalinacol Sin 2004;25(2):203-207.
  • [13]Xu JP(徐江平),LiL,Sun LS.Effects of ginkgolide on cerebral blood flow in dogs.J Chin Integr Med(中西医结合学报)2005;3(1):50-53(Chinese,English abstract).
  • [14]Lopes-Martins R,Catelli M,Araùjo C,Estato V,Cordeiro R,TibiricàE.Pharmacologica levidence of a role forplatelet activating factor as a modulator of vasomotor tone and blood pressure.Eur J Pharmacol 1996:308(3):287-294.
  • [15]Zhao G,He RR.The facilitating effect of atrial naaiuretic peptide on the carotid sinus baroreflex function.Chin J Physiol Sci 1993;9(1):68-75.
  • [16]Yi XL(易小林),Fan ZZ,He RR.An automatic system controlled by computer for carotid sinus perfusion.Chin J Appl Physiol (中国应用生理学杂志)1993;9(2):156-159(Chinese,English abstract).
  • [17]Kraske S,Cunningham JT,Hajduczok G,Chaplcan MW,Abboud FM,Wachtel RE.Mechanosensitive ion channels in putative aortic baroreceptor neurons.Am JPhysiol 1998;275(4Pt2):1497-1501.
  • [18]Cunninghanl JT,Wachtel RE,Abboud FM.Mechanical stimulation of neurites generates an inward current in putative aortic baroreceptor neurons in vitro.Brain Res 1997;757(1):149-154.
  • [19]Liu YX,Zhang H,Ma HJ,He RR.Cholecystokinin octapeptide inhibits carotid sinus baroreflex in anesthetized rats.Acta Physiol Sin(生理学报)2004;56(1):25-30.
  • [20]Schild JH,Clark JW,Hay M,Mendelowitz D,Andresen MC,Kunze DL.A-and C-type rat nodose sensory neurons:model interpretations of dynamic discharge characteristics.J Neurophysiol 1994; 71(6):2338-2358.
  • [21]Matsuda T,Bates JN,Lewis SJ,Abboud FM,Chapleau MW.Modulation of baroreceptor activity by nitric oxide and Snitrosocysteine.Circ Res 1995; 76(3):426-433.
  • [22]De Smet PA.Herbal remedies.N Engl J Med 2002; 347(25):2046-2056.
  • [23]Robert M,Matthew N.Cardiovascular Physiology.1th ed.Beijing:Health Science Asia,Elsevier Science,2002,189-190.
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