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Expression pattern of E2F6 in physical and chemical hypoxia-induced apoptosis

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Author:: SHU Bo(Laboratory of Molecular Cardiology of Institute of Health Sciences,Shanghai Jiao Tong University School of Medicine(SJTUSM) and Shanghai Institutes for Biological Sciences(SIBS),Chinese Academy of Sciences(CAS),Shanghai 200025,China)
YANG Wei-Wei(Laboratory of Molecular Cardiology of Institute of Health Sciences,SIBS,CAS and SJTUSM,Shanghai 200025,China)
YANG Huang-Tian(Laboratory of Molecular Cardiology of Institute of Health Sciences,SIBS,CAS and SJTUSM,Shanghai 200025,China)

Journal Title:: ACTA PHYSIOLOGICA SINICA

Issue:: Volume 60, Issue 01, 2008

DOI:

Key Word:: E2F6;apoptosis;physical hypoxia;cobalt chloride;desferrioxamine;H9c2 cells

Abstract： Apoptosis can be caused by hypoxia,a major factor during ischemic injury,in cardiomyocytes.However,the regulatory mechanisms underlying hypoxia-induced cardiomyocyte apoptosis have not yet been fully understood.E2F6,an identified E2F family member,has been demonstrated to repress DNA damage-induced apoptosis in our recent study.HoweveL it is unclear whether E2F6 is involved in hypoxia-induced apoptosis.In this study,we determined the expression property of E2F6 during hypoxia-induced apoptosis in H9c2 cells,a rat ventricular myoblast cell line.The results showed that physical hypoxia and chemical hypoxia-mimetic agents desferrioxamine(DFO)and cobalt chloride(CoCl2)induced apoptosis in H9c2 cells.Physical hypoxia-and CoCl2-induced apoptosis was accompanied with a downregulation of endogenous E2F6 mRNA expression,but not protein expression.DFO treatment resulted in a significant downregulation of both mRNA and protein expressions of endogenous E2F6.These results suggest that E2F6 may be involved in DFO-induced apoptosis,while it is less sensitive in physical hypoxia-and CoCl2-induced apoptosis in H9c2 cells.In addition,the apoptosis induced by DFO may share different pathways from that induced by physical hypoxia and CoCl2.

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