Changes of heart rate variability and impairment of learning and memory induced by cerebral ischemia/reperfusion in rats
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0371-0874.2007.01.007
- Key Word:
- 脑缺血再灌注;认知障碍;Morris水迷宫;心率变异性
Abstract： 本研究通过建立高脂缺血性脑损伤大鼠模型,进行水迷宫实验和心率变异性(heart rate variability,HRV)的功率谱分析,探讨脑损伤前后大鼠学习记忆的变化及缺血对自主神经的影响.23只成年雄性Wistar大鼠随机分为对照组(n=10)、高脂组(n=6)和高脂缺血组(n=7).高脂组和高脂缺血组大鼠均饲以高脂饲料制成高血脂大鼠模型.各组大鼠进行水迷宫实验后,高脂缺血组用双侧颈总动脉结扎(2-VO)法制作缺血再灌注模型,同时记录心电图.7 d后各组大鼠再次进行水迷宫实验和记录心电图,对HRV序列进行基于快速傅立叶转换(fast Fourier transformation,FFT)的功率谱分析.结果:(1)第一次水迷宫测试,3组大鼠空间探索实验和定位航行实验结果无统计学差异;第二次水迷宫实验,高脂缺血组与其它两组相比,空间探索实验中平台所在象限的记忆频度明显下降(P＜0.01),定位航行实验中平台所在象限的记忆频度显著下降(P＜0.01),10圆环记忆得分显著下降(P＜0.001),但高脂组与对照组相比无明显差异.(2)高脂缺血组缺血后心率持续下降:缺血时HRV中频段功率(0.2～0.6 Hz)呈现明显下降的趋势,高频段功率(0.6～2.5 Hz)缓慢下降,中频/高频功率比值明显下降(P＜0.05).(3)缺血7 d后高脂缺血组与高脂组相比,心率明显加快,HRV的中频段功率无显著变化,高频段功率明显下降,中频/高频功率比值显著增高(P＜0.05).结果表明,缺血过程中高脂缺血组大鼠的自主神经活动降低,交感神经活动相对于迷走神经活动减弱.缺血7 d后,由于海马区神经元对缺血敏感易受损,造成高脂缺血组大鼠学习记忆障碍,同时引发迷走神经活动下降,大鼠交感-迷走神经系统平衡失调.
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