Forced running enhances neurogenesis in the hippocampal dentate gyrus of adult rats and improves learning ability

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XU Wei-Ping()
SHAN Li-Dong()
GONG Shan()
CHEN Lin()
ZHANG Yue-Jin()
YIN Qi-Zhang()
JIANG Xing-Hong()
GUO Shi-Yu()
Journal Title:
Volume 58, Issue 05, 2006
Key Word:
hippocampus;neurogenesis;dentate gyrus;running;5-bromo-2-deoxyuridine;immunohistochemistry;maze learning;rats

Abstract: To investigate the effect of forced running in motor-driven wheel on neurogenesis in the hippocampal dentate gyrus (DG)of adult rats, 5-bromo-2-deoxyuridine (BrdU), a thymidine analog was applied to mark cell proliferation. Neuroepthelial stem cell protein (nestin) expression was used to identify neural stem/precursor cells. The BrdU- and nestin-positive cells were examined by immunohistochemical technique. The ability of learning was evaluated by Y-maze test to explore the functional role of the newborn cells in the DG after forced running. It was found that the number of BrdU- and nestin-positive cells in the DG in running groups was significantly increased compared to that in the control group (P<0.05). The effect of forced running on neurogenesis was intensitydependent. In addition, an improvement of learning ability in Y-maze test was observed after forced running. These findings suggest that forced running in motor-driven wheel could enhance neurogenesis in the hippocampal DG of adult rats and improve learning ability.

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