Inflammation of different tissues in spontaneously hypertensive rats

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Author:
SUN Li()
GAO Yue-Hong()
TIAN Deng-Ke()
ZHENG Jian-Pu()
ZHU Chun-Yun()
KE Yan()
BIAN Ka()
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
Volume 58, Issue 04, 2006
DOI:
Key Word:
spontaneously hypertensive rat;inflammation;kidney;liver;heart;brain

Abstract: The hypertension is one of chronic vascular diseases, which often implicates multiple tissues causing stroke, cardiac hypertrophy, and renal failure. A growing body of evidence suggests that inflammatory mechanisms are important participants in the pathophysiology of hypertension. In this study, the inflammatory status of these tissues (kidney, liver, heart, and brain) in spontaneously hypertensive rats (SHR) was analyzed and its molecular mechanism was explored. The tissues were dissected from SHR and agematched control Wistar-Kyoto (WKY) rats to investigate the abundance of inflammation-related mediators (IL-1β, TNFα, ICAM-1,iNOS, C/EBPδ and PPARγ). mRNA levels were determined by reverse transcription-polymerase chain reaction and protein expression was evaluated by Western blot. To evaluate the oxidative stress of tissues, carbonyl protein content and total antioxidant capacity of tissues were detected by spectrophotometry and ferric reduction ability power (FRAP) method. The results suggest that: (1)Expressions of inflammation-related mediators (IL-1β, TNFα, ICAM-1, iNOS, C/EBPδ and PPARγ) in SHR were higher compared with those in WKY rats except no evident increase of IL-1β mRNA in liver and brain in SHR. (2) Tissues in SHR contained obviously increased carbonyl protein (nmol/mg protein) compared to that in WKY rats (8.93±1.08 vs 2.27±0.43 for kidney, 2.23±0.23 vs 0.17±0.02 for heart, 13.42± 1.10 vs 5.72± 1.01 for brain, respectively, P<0.05). However, no evident difference in the amount of carbonyl protein in liver was detected between SHR and WKY rats. (3) Total antioxidant capacities of kidney, liver, heart and brain were markedly lower in SHR than that in WKY rats (P<0.05). Thus, the present data reveal a higher inflammatory status in the important tissues in SHR and indicate that inflammation might play a potential role in pathogencsis of hypertension and secondary organ complications.

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