Identification and function of the growth hormone gene in Rongjiang pig of China

( views:114, downloads:0 )
LI Jing()
RAN Xue-Qin()
WANG Jia-Fu()
Journal Title:
Volume 58, Issue 03, 2006
Key Word:
Rongjiang pig;growth hormone;gene

Abstract: Growth of animal is largely regulated by growth hormone (GH). In this study, the GH gene was isolated and cloned from the genomic DNA library from Rongjiang pig, a Chinese local swine, using polymerase chain reaction technique. The complete nucleotide sequence of a 1.903 kb genomic fragment containing Rongjiang swine GH gene has been determined. The GH gene contained five exons and four introns similar to the GH genes of other mammalians and exhibited 97%~99% identity to the GH genes of the four western meat-type breeds and nine Chinese local pigs. Polymorphism of GH genes was analyzed by using the restriction enzymes Dde I, Nar I and BsmN I in four western meat-type breeds and ten Chinese local pigs. Five polymorphic restriction sites, with Dde I at the base 622 (G/A) in exon 2 and 274 (T/C) in 5'-flank, with Nar I at 631 (G/A) in exon 2, and with BsmN I at the base 841 (T/C) in intron 2 and 1 358 (A/G) in exon 4, were identified. The polymorphic restriction site at 1 358 (A/G) leaded to the GH mature protein of Rongjiang pig differing from that of four western meat-type breeds and eight Chinese local breeds at the residue Val108 substituted by Ile108.According to the crystal structure of human GH mature protein, this Ile108 substitution might result in a lower affinity of GH for its receptor in Rongjiang breed.

  • [1]Sterle JA,Cantley TC,Lamberson WR,Lucy MC,Gerrard DE,Matteri RL,Day BN.Effects of recombinant porcine somatotropin on placental size,fetal growth,and IGF-Ⅰ and IGF-Ⅱ concentrations in pigs.J Anim Sci 1995;73:2980-2985
  • [2]Ran XQ,Li WS,Lin HR.Stimulatory effects of gonadotropinreleasing hormone and dopamine on growth hormone release and growth hormone mRNA expression in Epinephelus coioides.Acta Physiol Sin (生理学报) 2004;56:644-650
  • [3]Waters MJ,Hoang HN,Fairlie DP,Pelekanos RA,Brown RJ.New insights into growth hormone action.J Mol Endocrinol 2006;36:1-7
  • [4]MacLeod JN,Lee AK,Liebhaber SA,Cooke NE.Developmental control and alternative splicing of the placentally expressed transcripts from the human growth hormone gene cluster.J Biol Chem 1992;267:14219-14226
  • [5]Golos TG,Duming M,Fisher JM,Fowler PD.Cloning of four growth hormone/chorionic somatomammotropin-related complementary deoxyribonucleic acids differentially expressed during pregnancy in the rhesus monkey placenta.Endocrinology 1993;133:1744-1752
  • [6]Yerle M,Mansais Y,Thomsen PD,Gellin J.Location of the porcine hormone gene to chromosome 12p1.2~p1.5.Anim Genet 1993;24:129-131
  • [7]Cheng WTK,Lee CH,Hung CM,Chang ITJ,Chen CM.Growth hormone gene polymorphisms and growth performance traits in Duroc,Landrace and Tao-Yuan pigs.Theriogenology 2000;54:1225-1237
  • [8]Vize PD,Wells JR.Isolation and characterization of the porcine growth hormone gene.Gene 1987;55:339-344
  • [9]Nielson VH,Larsen NJ.Restriction fragment length polymorphisms at the growth hormone gene in pigs.Anim Genet 1991;22:291-294
  • [10]Kirkpatrick BW.Hae Ⅱ and Msp I polymorphisms are detected in the second intron of the porcine growth hormone gene.Anim Genet 1992;23:180-181
  • [11]Larsen NJ,Nielson VH.Apa I and Cfo I polymorphisms in the porcine growth hormone gene.Anim Genet 1993;24:71
  • [12]Marques PX,Pereira M,Marques MR,Santos IC,Belo CC,Renaville R,Cravador A.Association of milk traits with SSCP polymorphisms at the growth hormone gene in the Serrana goat.Small Ruminant Res 2003;50:177-185
  • [13]Zhou GL,Jin HG,Chen L,Guo SL,Zhu Q,Wu YH.Association of genetic polymorphism in GH gene with milk production traits in Beijing Holstein cows.J Biosci 2005;30:595-598
  • [14]Edwards DB,Tempelman RJ,Bates RO.Evaluation of Durocvs.Pietrain-sired pigs for growth and composition.J Anim Sci 2006;84:266-275
  • [15]Sambrook J,Russell DW.Molecular Cloning:A Laboratory Mannual.3rd ed.New York:Cold Spring Harbor Laboratory Press,2001
  • [16]Altschul SF,Gish W,Miller W,Myers EW,Lipman DJ.Basic local alignment search tool.J Mol Biol 1990;215:403-410
  • [17]Schwede T,Kopp J,Guex N,Peitsch MC.SWISS-MODEL:An automated protein homology-modeling server.Nucleic Acids Res 2003;31:3381-3385
  • [18]De Vos AM,Ultsch M,Kossiako VAA.Human growth hormone and extracellular domain of its receptor:crystal structure of the complex.Science 1992;255:306-312
  • [19]Walsh STR,Jevitts LM,Sylvester JE,Kossiakoff AA.Site2 binding energetics of the regulatory step of growth hormoneinduced receptor homodimerization.Protein Sci 2003;12:1960-1970
  • [20]Woychik RP,Camper SA,Lyons RH,Horowitz S,Goodwin EC,Rottman FM.Cloning and nucleotide sequencing of the bovine growth hormone gene.Nucleic Acids Res 1982;10:7197-7210
  • [21]Warwick JM,Wallis OC,Wallis M.Cloning,sequence and expression in Escherichia coli of cDNA for ovine pregrowth hormone.Biochim Biophys Acta 1989;1008:247-250
  • [22]Yamano Y,Oyabayashi K,Okuno M,Yato M,Kioka N,Manabe E,Hashi H,Sakai H,Komano T,Utsumi K,Iritani A.Cloning and sequencing of cDNA that encodes goat growth hormone.FEBS Lett 1988;228:301-304
  • [23]Roskam W,Rougeon F.Molecular cloning and nucleotide sequence of the human growth hormone structural gene.Nucleic Acids Res 1979;7:305-320
  • [24]Lioupis A,Wallis OC,Wallis M.Cloning and characterization of the gene encoding red deer (Cervus elaphus) growth hormone:implications for the molecular evolution of growth hormone in artiodactyls.J Mol Endocrinol 1997;19:259-266
  • [25]Wallis CO,Wallis M.Molecular evolution of growth hormone (GH) in cetartiodactyla:cloning and characterization of the gene encoding GH from a primitive ruminant,the chevrotain (Tragulus javanicus).Gen Comp Endocrinol 2001;123:62-72
  • [26]Wells JA,Cunningham BC,Fuh G,Lowman HB,Bass SH,Mulkerrin MG,Ultsch M,de Vos AM.The molecular basis for growth hormone-receptor interactions.Rec Prog Horm Res 1993;48:253-275
  • [27]Walsh STR,Sylvester FD,Kossiakoff AA.The high-and lowaffinity receptor binding sites of growth hormone are allosterically coupled.Proc Natl Acad Sci USA 2004;101:17078-17083
  • [28]Brian CC,James AW.Comparison of a structural and a functional epitope.J Mol Bio 1993;234:554-563
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615