You Position: Home > Paper
Protective effect of sevoflurane preconditioning on oxygen-glucose deprivation injury in rat hippocampal slices: the role of mitochondrial KATP channels
( views:226, downloads:25 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0371-0874.2006.03.002
- Key Word:
- 七氟醚;脑损伤;顺向群峰电位;线粒体内膜ATP敏感钾通道
Abstract： 利用离体海马脑片缺氧无糖(oxygen-glucose deprivation,OGD)损伤模型,探讨七氟醚预处理对神经细胞的保护作用及该作用与线粒体内膜ATP敏感钾通道(mitochondrial ATP-sensitive potassium channels,mitoKATPchannels)的关系,随机将脑片用2%、4%、6%七氟醚,以及6%七氟醚复合mitoKATP通道阻滞剂5-羟基奎酸盐(5-hydroxydecanoic acid,5-HD)预处理30 min,观察OGD损伤14 min复氧1 h期间顺向群峰电位(orthodromic population spike,OPS)的变化,并应用透射电镜观察细胞超微结构的改变.结果表明,与单纯OGD组相比,七氟醚预处理可使海马脑片OPS消失时间明显延长(P＜0.01),使OPS明显恢复,其中4%、6%七氟醚组的恢复率均为71.4%(P＜0.05 vs OGD),相应恢复程度为(61.0±42.3)%和(78.7±21.1)%(P＜0.01),而且6%七氟醚的保护作用可被5-HD取消.OGD组的海马CA1区锥体细胞明显水肿,核膜皱缩、破裂,染色质聚集,线粒体肿胀畸形,嵴断裂或消失,而4%和6%七氟醚组仅见海马CA1区锥体细胞轻度水肿,核膜皱缩不明显,染色质均匀,线粒体轻度肿胀.结果提示,七氟醚预处理对大鼠海马脑片OGD损伤有一定的保护作用,且七氟醚对神经细胞的保护作用与激活mitoKATP通道有关.
- [1]Wise Faberowski L,Raizada MK,Sumners C.Desflurane and sevoflurane attenuate oxygen and glucose deprivation-induced neuronal cell death.[J].2003,15(3).
- [2]Maurer M,Kapinya KJ,Waschke KF,etc.Tolerance against ischemic neuronal injury can be induced by volatile anesthetics and is inducible NO synthase dependent.[J].2002,33(7).
- [3]M W, Crawford,J, Lerman,V, Saldivia,etc.Hemodynamic and organ blood flow responses to halothane and sevoflurane anesthesia during spontaneous ventilation.[J].1992,75.
- [4]Calkins CM,Heimbach JK,Pomerantz BJ,etc.Selective mitochondrial KATP channel opening controls human myocardial preconditioning: too much of a good thing?[J].2000,128(2).
- [5]G J, Gross,J A, Auchampach.Role of ATP dependent potassium channels in myocardial ischaemia.[J].1992,26.
- [6]Toller Wolfgang G.,Montgomery Matthew W.,Pagel Paul S.,etc.Isoflurane-enhanced Recovery of Canine Stunned Myocardium[J].1999.
- [7]Gross GJ.The role of mitochondrial KATP channels in cardioprotection.[J].2000,95(4).
- [8]Pavlik R,McCadden T,Kass IS,etc.Sevoflurane improves electrophysiological recovery of rat hippocampal slice CA1 pyramidal neurons after hypoxia.[J].2002,14(4).
- [9]Bajgar R,Kowaltowski AJ,Seetharaman S,etc.Identification and properties of a novel intracellular (mitochondrial) ATP-sensitive potassium channel in brain.[J].2001,276(36).
- [10]Roewer N,Kehl F,Payne RS,etc.Sevoflurane-induced preconditioning of rat brain in vitro and the role of K(ATP) channels.[J].2004,1021(1).
- [11]D K, Cope,W K, Impastato,M V, Cohen,etc.Volatile anesthetics protect the ischemic rabbit myocardium from infarction.[J].1997,86.